烟草科技2025，Vol.58Issue(10)：67-72,112,7.DOI:10.16135/j.issn1002-0861.2024.0929
嘧菌酯对烟草立枯丝核菌菌核形成及致病力的影响
Effects of azoxystrobin on sclerotial formation and pathogenicity of Rhizoctonia solani in tobacco
摘要
Abstract
To explore the potential of azoxystrobin in controlling target sport diseases of tobacco caused by Rhizoctonia solani,30 strains isolated from different regions in Guizhou Province were used as test materials.The effects of azoxystrobin on sclerotial formation were analyzed using the plate incorporation method and Biolog FF microplate technology,while its effects on pathogenicity were analyzed using the detached leaf assay.The results showed that all 30 strains were able to form sclerotia on PDA medium.The LK4 strain produced the most sclerotia,demonstrating the strongest sclerotial formation ability.Compared to the PDA medium without azoxystrobin,8 strains exhibited delayed sclerotial formation,reduced maturity and lower sclerotia yield,while 22 strains failed to form sclerotia on the PDA medium containing azoxystrobin(5 μg/mL).Without azoxystrobin stress,the LK4 strain could metabolize all substrates on the FF microplate expect sebacic acid,and could form sclerotia when induced by 38 substrates including D-cellobiose,β-cyclodextrin,and dextrin.Azoxystrobin stress(5 μg/mL)increased the metabolic capacity of the LK4 strain towards salicin and arbutin in the FF microplate.However,the metabolic capacity towards 90 substrates decreased,excluding L-fucose,L-sorbose,γ-aminobutyric acid and D-lactic acid methyl ester.Furthermore,none of the substrates induced sclerotial formation in the LK4 strain under azoxystrobin stress.Results from the detached leaf assay experiment showed that lesions formed on the leaves within 7 days following inoculation with test strains without azoxystrobin stress.After culturing the strains on a PDA medium containing azoxystrobin(5 μg/mL)for 4 days and inoculating them onto detached leaves,25 of the tested strains failed to form lesions on the leaves after 7 days.The 5 strains that did form lesions had significantly smaller lesion diameters compared to those without azoxystrobin stress.关键词
嘧菌酯/立枯丝核菌/菌核/致病力/化学防治Key words
Azoxystrobin/Rhizoctonia solani/Sclerotium/Pathogenicity/Chemical control分类
植物保护学引用本文复制引用
向立刚,汪汉成,蔡刘体,陈丽莉,张文建,孟建玉,田维强,陈代利..嘧菌酯对烟草立枯丝核菌菌核形成及致病力的影响[J].烟草科技,2025,58(10):67-72,112,7.基金项目
国家自然科学基金项目"基于转录组学和表型组学的嘧菌酯抑制烟草立枯丝核菌菌核形成机理的研究"(31960550) （31960550）
贵州省科技厅基金项目"烟草靶斑病病际微生态及基于微生态的防治技术研究"(黔科合基础-ZK[2021]重点036) （黔科合基础-ZK[2021]重点036）
贵州省人才项目"贵州省'百层次'创新型人才"(GCC[2022]028-1、GCC[2023]108) （GCC[2022]028-1、GCC[2023]108）
中国烟草总公司科技项目"基于叶际微生态的烟区多病害复合侵染机制研究"[110202101048(LS-08)] （LS-08）
中国烟草总公司科技重大专项项目"烟草主要叶斑类病害监测预警及绿色防控技术研究与应用"[110202001035(LS-04)]. （LS-04）
