中国病理生理杂志2025，Vol.41Issue(10)：1926-1937,12.DOI:10.3969/j.issn.1000-4718.2025.10.007
长链非编码RNA LINC01615通过上调TEAD2表达促进头颈鳞癌细胞恶性表型
Long noncoding RNA LINC01615 promotes malignant phenotypes of head and neck squamous cell carcinoma cells by up-regulating TEAD2 expres-sion
摘要
Abstract
AIM:To investigate the biological functions and molecular mechanisms of long noncoding RNA LINC01615 in head and neck squamous cell carcinoma(HNSCC)cells.METHODS:Transcriptome sequencing data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases were used to analyze the expres-sion level of LINC01615 in HNSCC cells and its correlation with patient survival.RT-qPCR was used to detect the expres-sion levels of LINC01615 in HNSCC and normal control cells.An siRNA-mediated LINC01615 knockdown HNSCC cell model was established,and high-content screening cell counting,ATP and CCK8 assays were performed to analyze cell proliferation.Transwell assays were conducted to assess cell migration and invasion.Bioinformatics analysis was em-ployed to predict potential target genes of LINC01615 and the biological processes and signaling pathways involved.RT-qPCR and Western blot were used to validate the regulatory effect of LINC01615 on the candidate target gene TEAD2.Transcriptome data from TCGA and GEO databases were analyzed to determine the expression pattern of TEAD2 in HN-SCC.Functional cell experiments were performed to investigate the impact of TEAD2 knockdown on HNSCC proliferation,migration,and invasion.Rescue experiments were conducted to examine whether LINC01615 influenced the malignant phenotypes(proliferation,migration,and invasion)of HNSCC cells by regulating TEAD2 expression.RESULTS:The expression levels of LINC01615 were significantly higher in HNSCC tissues and cells than those in normal control tissues and cells,respectively(P<0.01).Knockdown of LINC01615 significantly inhibited HNSCC proliferation,migration,and invasion(P<0.01).Bioinformatics analysis identified 134 candidate target genes of LINC01615,which were primarily en-riched in tumor-related biological processes and signaling pathways,including angiogenesis,regulation of endothelial cell proliferation,regulation of cell migration,HPV infection,Hippo signaling pathway,and PI3K-Akt signaling pathway.Knockdown of LINC01615 led to a significant decrease in TEAD2 expression in HNSCC cells(P<0.01).Functional cell studies demonstrated that TEAD2 knockdown suppressed HNSCC proliferation,migration,and invasion,whereas TEAD2 overexpression reversed the inhibitory effects of LINC01615 knockdown on these malignant phenotypes.CONCLUSION:LINC01615 is upregulated in HNSCC tissues and cells,functioning as an oncogene.Mechanistic studies reveal that LINC01615 promotes HNSCC proliferation,migration,and invasion by upregulating TEAD2,a key transcription factor in the Hippo signaling pathway.These findings may provide a novel potential biomarker for the clinical diagnosis and treat-ment of HNSCC.关键词
长链非编码RNA/LINC01615/头颈部鳞状细胞癌/TEAD2基因Key words
long noncoding RNA/LINC01615/head and neck squamous cell carcinoma/TEAD2 gene分类
临床医学引用本文复制引用
吴博,张鹏辉,高宁,张慧敏,邢可尧,屈沫怡,宋茹..长链非编码RNA LINC01615通过上调TEAD2表达促进头颈鳞癌细胞恶性表型[J].中国病理生理杂志,2025,41(10):1926-1937,12.基金项目
国家自然科学基金资助项目(No.32360162) （No.32360162）
2024年陕西省大学生创新训练计划项目(No.S202410719134) （No.S202410719134）
