中国病理生理杂志2025，Vol.41Issue(10)：1972-1982,11.DOI:10.3969/j.issn.1000-4718.2025.10.012
督脉推法对孤独症谱系障碍模型小鼠青少年期海马内C1q/C3介导小胶质细胞突触修剪功能的影响
Effect of Du Meridian pushing on synaptic pruning function of microglia mediated by C1q/C3 in hippocampus of adolescent mice with autism spectrum disorder
摘要
Abstract
AIM:To investigate whether direct stimulation of the Du Meridian improves autism-like behaviors in mice by modulating synaptic pruning.METHODS:Pregnant mice received intraperitoneal injections of valproic acid(VPA)or saline on gestational day 12.5.Offspring from saline-treated mice were assigned to the saline group.Offspring from VPA-treated mice were randomly divided into the model(VPA)group and the VPA-model-DuMaiTuiFa(VPA-DMTF)group.Each group included five mice.The VPA-DMTF group received direct stimulation along the Du Mai at 80 times per minute,10 minutes per session,twice daily for 21 days.After treatment,behavioral tests were conducted,in-cluding the three-chamber test for social interaction and the open field test for anxiety-like behavior.Hippocampal tissue was collected for analysis.Golgi staining was used to assess dendritic spine density.Immunofluorescence staining for post-synaptic denisty protein(PSD95)and synapsin 1(SYN1)was performed to evaluate synaptic protein expression.Staining for ionized calcium-binding adapter molecule 1(IBA1)and CD68 was used to assess microglial phagocytosis.Western blot analysis was conducted to evaluate hippocampal expression levels of PSD95,SYN1,IBA1,complement component 1,q subcomponent(C1q),complement component 3(C3),and complement receptor 3(CR3).RESULTS:Compared with the saline group,VPA mice showed reduced social behavior and increased anxiety(P<0.05).The expression levels of IBA1,PSD95,and SYN1 were significantly increased(P<0.05),whereas C1q,C3,and CR3 were significantly de-creased(P<0.05).Microglial phagocytosis declined.Immunofluorescence analysis showed increased levels of synaptic proteins(PSD95 and SYN1)in the VPA group(P<0.05).Golgi staining revealed a higher dendritic spine density and an increased proportion of immature dendritic spines(P<0.05).Compared with the VPA group,VPA-DMTF mice showed improved behavior,reduced IBA1,PSD95,and SYN1 levels(P<0.05),and increased expression of C1q,C3,and CR3(P<0.05).Microglial phagocytosis was enhanced,and dendritic spine number was reduced.CONCLUSION:Direct stimulation of the Du Mai alleviates autism-like behaviors in mice.This effect may be mediated by upregulation of comple-ment proteins C1q and C3,which enhance microglia-mediated synaptic pruning and reduce synaptic overabundance.关键词
直推督脉/孤独症谱系障碍/小胶质细胞/突触修剪/补体Key words
direct stimulation of the Du Meridion/autism spectrum disorder/microglia/synaptic pruning/complement分类
医药卫生引用本文复制引用
陈依楠,赵莎彤,胡明岸,马梦娟,李武,冯祥,李江山,张宇星..督脉推法对孤独症谱系障碍模型小鼠青少年期海马内C1q/C3介导小胶质细胞突触修剪功能的影响[J].中国病理生理杂志,2025,41(10):1972-1982,11.基金项目
中国博士后科学基金面上项目(No.2024M760895) （No.2024M760895）
湖南省自然科学基金青年项目(No.2024JJ6341) （No.2024JJ6341）
湖南中医药大学校级国自然预研项目(No.2024XJYY07) （No.2024XJYY07）
湖南中医药大学研究生科研创新项目(No.2024CX159) （No.2024CX159）
