中国病理生理杂志2025，Vol.41Issue(10)：1983-1992,10.DOI:10.3969/j.issn.1000-4718.2025.10.013
化瘀祛痰方含药血清通过内质网应激途径对油酸诱导的HepG2细胞脂肪酸从头合成的影响
Effects of Huayu-Qutan formula-medicated serum on de novo fatty acid synthesis in HepG2 cells induced by oleic acid via endoplasmic reticulum stress
摘要
Abstract
AIM:The study aims to investigate the effects of Huayu-Qutan formula(HYQT)-medicated serum on oleic acid-induced lipid damage and endoplasmic reticulum stress(ERS)in HepG2 cells,and to explore the mecha-nism underlying the prevention and treatment of lipid metabolism disorders by HYQT.METHODS:The HepG2 cells were treated with various concentrations of oleic acid,and CCK8 assay,oil red O staining and ELISA were used to identify the optimal treatment concentration and time of oleic acid and HYQT-medicated serum.Moreover,the cells were divided into control,model,thapsigargin(Tg),and Tg+HYQT groups.Cellular lipid deposition was measured using oil red O staining,while triglyceride(TG)and free fatty acid(FFA)levels were assessed by ELISA.Transmission electron micros-copy was used to examine endoplasmic reticulum structures,and RT-qPCR and Wes fully automated protein quantification analysis system were used to measure the mRNA and protein expression of glucose-regulated protein 78(GRP78),sterol regulatory element binding protein 1c(SREBP1c),acetyl coenzyme A carboxylase(ACC1),fatty acid synthase(FAS)and stearoyl coenzyme A desaturase 1(SCD1)in different groups.RESULTS:Significant lipid deposition was induced in HepG2 cells after treatment with 1 000 µmol/L oleic acid,while treatment with serum containing 10%HYQT for 48 h was found to be optimal.Compared with control group,the cells in model group showed significant deposition of oil red O-stained lipid droplets in the cytoplasm,associated with endoplasmic reticulum expansion,ERS,and nuclear condensa-tion.The TG and FFA levels,and the mRNA and protein expression levels of GRP78,SREBP1c,ACC1,FAS and SCD1 were increased significantly(P<0.01).Compared with model group,the cells treated with HYQT-mediated serum showed marked decrease in the number of lipid droplets in the cytoplasm,with restored endoplasmic reticulum morphology.The TG and FFA levels were significantly reduced(P<0.01),and the mRNA and protein levels of factors related to ERS and de novo fatty acid synthesis were markedly decreased(P<0.01).Treatment with Tg,an ERS agonist,led to greater accu-mulation of cytoplasmic lipid droplets and increased endoplasmic reticulum expansion.Marked variations in the morpholo-gy and size of the endoplasmic reticulum were observed,with fusion and clustering of vacuoles.The TG and FFA levels,and the expression of ERS-and fatty acid synthesis-related factors were increased(P<0.01).Compared with Tg group,the cells treated with Tg+HYQT showed reduced number of cytoplasmic lipid droplets,attenuated endoplasmic reticulum dilation,and decreased number and volume of vacuoles,while the TG and FFA levels and the expression of ERS-and fatty acid synthesis-associated factors were significantly decreased(P<0.01).CONCLUSION:Serum containing HYQT alle-viates oleic acid-induced lipid damage in HepG2 cells by inhibiting ERS-induced de novo synthesis of fatty acids.关键词
化瘀祛痰方/HepG2细胞/内质网应激/脂肪酸合成Key words
Huayu-Qutan formula/HepG2 cells/endoplasmic reticulum stress/fatty acid synthesis分类
中医学引用本文复制引用
杜莹,宋囡,吕美君,王莹,杨莹,王杰,贾连群..化瘀祛痰方含药血清通过内质网应激途径对油酸诱导的HepG2细胞脂肪酸从头合成的影响[J].中国病理生理杂志,2025,41(10):1983-1992,10.基金项目
国家自然科学基金青年基金项目(No.82405219) （No.82405219）
辽宁省科技计划联合计划(基金)项目(No.2023-MSLH-186) （基金）
辽宁省教育厅面上项目(No.LJKMZ20221316) （No.LJKMZ20221316）
