中国病理生理杂志2025，Vol.41Issue(10)：2009-2015,7.DOI:10.3969/j.issn.1000-4718.2025.10.016
六味地黄汤通过Notch信号通路调控卵巢早衰小鼠卵巢功能
Liuwei-Dihuang decoction modulates ovarian function in POF mice via the Notch signaling pathway
摘要
Abstract
AIM:To explore the therapeutic effects of Liuwei-Dihuang decoction(LD)on ovarian function in pre-mature ovarian failure(POF)mice,and to uncover the role of the Notch signaling pathway in its mechanism.METHODS:A mouse model of POF was constructed through intraperitoneal injection of cyclophosphamide/busulfan.Subsequently,the mice were randomly divided into six groups(n=12 per group):control,POF model,low-dose LD(LD-L),medium-dose LD(LD-M),high-dose LD(LD-H),and positive drug(0.7 mg/kg diethylstilbestrol)groups.The ovarian histopathologi-cal alterations were assessed by hematoxylin-eosin(HE)staining.Serum levels of follicle-stimulating hormone(FSH),anti-Müllerian hormone(AMH),and estradiol(E2)were quantified via ELISA assays.Immunohistochemistry(IHC)was conducted to compare the expression level of cell proliferation marker Ki-67 in mouse ovarian tissues among the groups.The expression of proteins involved in the Notch pathway components[Notch1,Notch2,Jagged1(JAG-1),and hairy and enhancer of split 1(Hes1)]was determined via IHC and Western blot,while the corresponding mRNA levels of Notch1,Notch2,JAG-1,JAG-2,Hes1,and Hes5 were quantified through the qPCR analysis.RESULTS:Ovarian weight was significantly higher,and the number of atretic follicles was significantly lower in the LD-H and positive drug groups com-pared to the model group(P<0.05).However,serum FSH levels were decreased(P<0.01),whereas those of E2 and AMH were elevated in both treatment groups compared with the model group(P<0.01).In the LD-H group,the expres-sion of key molecules of the Notch pathway components was increased at the protein and mRNA level compared with the model group(P<0.01).CONCLUSION:LD ameliorates ovarian dysfunction in POF mice via activating the Notch sig-naling pathway.关键词
卵巢早衰/卵泡发育/六味地黄汤/Notch通路Key words
premature ovarian failure/follicular development/Liuwei-Dihuang decoction/Notch signaling pathway分类
医药卫生引用本文复制引用
梁夏,李妮娜,叶海峰,杨晓婷,胡燎燎..六味地黄汤通过Notch信号通路调控卵巢早衰小鼠卵巢功能[J].中国病理生理杂志,2025,41(10):2009-2015,7.基金项目
衢州市科技攻关项目(No.2023K249) （No.2023K249）
