中国草地学报2025，Vol.47Issue(10)：1-10,10.DOI:10.16742/j.zgcdxb.20240471
红三叶独脚金内酯TpD27基因表达与功能分析
Expression and Functional Analysis of the Strigolactones-Related Gene TpD27 in Red Clover
摘要
Abstract
The D27(DWARF27)gene encodes an iron-containing β-carotene isomerase essential for strigolactone biosynthesis.In this study,the TpD27 gene was cloned from Trifolium pratense'Minshan'using RT-PCR.Bioinformatics analysis revealed that the full-length coding sequence of TpD27 was 792 bp,encoding a 264-amino acid protein with a predicted molecular weight of 29.71 kDa,an isoelectric point of 8.76,and an instability index of 51.30,indicating that it was an unstable protein.Subcellular localization prediction suggested that TpD27 may be localized in the chloroplasts.The secondary structure of TpD27 was mainly composed of α-helix(33.71%)and random coil(55.68%),consistent with its predicted tertiary structure.Phylogenetic analysis showed that TpD27 clusters closely with MtD27 from Medicago truncatula,indicating a close evolutionary relationship between the two species.Spatiotemporal expression profiling revealed tissue-specific expression of TpD27,with the highest transcript levels in flowers and the lowest in roots.Under abiotic stress conditions,TpD27 expression was significantly downregulated in response to cadmium,salt,and drought treatments.Under low-temperature stress,its expression followed a dynamic pattern:initial downregulation,subsequent upregulation,and eventual decline.The recombinant expression vector pCAMBIA2300-TpD27 was successfully introduced into wild-type Arabidopsis thaliana via Agrobacterium-mediated floral-dip transformation.Phenotypic analysis demonstrated that the transgenic Arabidopsis thaliana exhibited a significantly reduced number of branches compared to the wild-type plants.关键词
红三叶/TpD27基因/表达分析/基因克隆/遗传转化Key words
Trifolium pratense/TpD27 gene/Expression analysis/Gene cloning/Genetic transformation分类
农业科技引用本文复制引用
杨丹,牛奎举..红三叶独脚金内酯TpD27基因表达与功能分析[J].中国草地学报,2025,47(10):1-10,10.基金项目
青海理工大学(筹)"昆仑英才"人才引进科研项目(2023-QLGKLYCZX-013) （筹）
青海理工学院论文延伸项目(202302lwys011) （202302lwys011）
