中国机械工程2025，Vol.36Issue(10)：2359-2368,10.DOI:10.3969/j.issn.1004-132X.2025.10.025
集成流体与温度控制的液滴型数字PCR微流控芯片设计与应用
Design and Applications of Droplet-based Digital PCR Microfluidic Chip with Integrated Fluid and Temperature Control
摘要
Abstract
Digital PCR(dPCR)had emerged as a vital tool in molecular biology and clinical diagnos-tics due to the high sensitivity,accuracy,and absolute quantification capabilities.However,challenges had persisted regarding integration level,sample volume requirements and fluid control.An integrated droplet-based dPCR microfluidic chip incorporating a rapid valve system and automated heating system was pre-sented,along with optimized fabrication processes herein.The results show that the chip may generate droplets efficiently and uniformly,with a maximum generation frequency of 5.132 kHz and a droplet diam-eter CV(the coefficient of variation)of only 1.67%.Rapid fluid control within 0.1s is enabled by the de-formable valve,while temperature ramping rates of 1.2℃/s with temperature variations within±0.6℃are achieved by the integrated on-chip thermal cycling system.Fluid evaporation is effectively prevented by an improved PDMS formulation,and the accuracy of 99.7%is achieved by the droplet recognition algorithm.关键词
微流控芯片/微液滴/数字聚合酶链式反应/形变阀/集成化Key words
microfluidic chip/microdroplet/digital polymerase chain reaction(PCR)/deformable valve/integration分类
化学引用本文复制引用
何春华,姚金辉,聂磊,廖广兰,史铁林,刘智勇..集成流体与温度控制的液滴型数字PCR微流控芯片设计与应用[J].中国机械工程,2025,36(10):2359-2368,10.基金项目
中央高校基本科研业务费专项资金(2024JYCXJJ031) （2024JYCXJJ031）
华中科技大学"交叉研究支持计划"(2024JCYJ070) （2024JCYJ070）
