中国马铃薯2025，Vol.39Issue(2)：81-90,10.DOI:10.19918/j.cnki.1672-3635.2025.02.001
马铃薯育种材料PVX抗性基因的检测与鉴定
Detection and Identification of Potato Virus X(PVX)Resistance Genes in Potato Breeding Materials
摘要
Abstract
Potato viruses are the fundamental cause of potato variety degeneration.Identifying SCAR molecular markers associated with PVX resistance genes in potato breeding materials at home and abroad is crucial for screening and evaluating germplasm resistant to PVX.Utilizing 76 potato varieties(lines)as test materials,this study analyzed SCAR markers linked to PVX resistance genes Rx1,Rx2,and the hypersensitivity gene Nb.Concurrently,under controlled conditions,materials were artificially inoculated with PVX,and viral infection status was confirmed via enzyme-linked immunosorbent assay(ELISA).Molecular marker analysis revealed that except for three materials lacking detectable markers for any PVX resistance genes,all other tested varieties(lines)possessed one to four markers associated with PVX resistance genes.Materials carrying Rx genes and Nb gene accounted for 29%and 15%of the total,respectively,while 52%contained both Rx and Nb genes.Following screening,23 potato varieties(lines)were artificially inoculated with PVX and confirmed by ELISA.Among these,19 varieties(lines)exhibited high resistance to PVX,characterized by the absence of phenotypic symptoms and negative ELISA results.Three materials('Miras','Pamyati Kunaeva'and'KC-4')demonstrated tolerance to PVX,as PVX was detected in leaf tissues,yet no phenotypic symptoms were observed.The highly PVX-resistant varieties(lines)identified could be incorporated into subsequent potato breeding programs,providing a theoretical foundation for molecular marker-assisted selection in developing PVX-resistant potato varieties domestically and internationally.关键词
马铃薯X病毒/马铃薯品系/分子标记/病毒侵染/抗病毒性Key words
potato virus X/breeding line/molecular marker/infection/resistance分类
农业科技引用本文复制引用
龚丽娟,Moldir Azhimakhan,李学洋,孙莎莎,Vadim Khassanov,胡柏耿..马铃薯育种材料PVX抗性基因的检测与鉴定[J].中国马铃薯,2025,39(2):81-90,10.基金项目
2023年内蒙古中哈合作"一带一路"广适马铃薯新品种选育项目(2023KJHZ0011). （2023KJHZ0011）
