中国马铃薯2025，Vol.39Issue(2)：131-139,9.DOI:10.19918/j.cnki.1672-3635.2025.02.008
黑龙江省克山县马铃薯早疫病病原菌鉴定及生防研究
Identification and Biocontrol of Potato Early Blight in Keshan County,Heilongjiang Province
摘要
Abstract
Potato early blight is a leaf disease caused by Alternaria ssp.To determine the pathogen species causing potato early blight in Keshan County,Heilongjiang Province,pathogens were isolated from 15 diseased leaf samples collected across eight fields using a tissue isolation method and identified based on morphological characteristics and molecular analyses.Antagonistic strains against the early blight pathogens were screened by plate confrontation(dual-culture)assays,and their antifungal activity was evaluated.In total,16 pathogen isolates were obtained from diseased leaves:14 isolates of Alternaria alternata and 2 of A.solani.A.alternata(TJ-16)was small-spored,whereas A.solani(TJ-29)was large-spored.One strain,Bacillus velezensis ZK-18,showed strong inhibitory effects against potato early blight,markedly suppressing pathogen mycelial growth and reducing disease on detached potato leaves,with inhibition rates of 50.00%(TJ-16)and 53.59%(TJ-29),respectively.These results would provide new ideas for the biological control of potato foliar diseases.关键词
马铃薯早疫病/鉴定/贝莱斯芽孢杆菌/拮抗作用Key words
potato early blight/identification/bacillus velezensis/antagonism分类
农业科技引用本文复制引用
王鹏,高艳玲,邸桂俐,韩树鑫,张抒,范国权..黑龙江省克山县马铃薯早疫病病原菌鉴定及生防研究[J].中国马铃薯,2025,39(2):131-139,9.基金项目
黑龙江省农业科学院创新工程项目(CX23YQ06) （CX23YQ06）
黑龙江省自然科学基金资助项目(LH2024C104) （LH2024C104）
黑龙江省省属科研院所科研业务费项目(CZKYF2024-1-A005 （）
CZKYF2024-1-C024) （）
黑龙江省农业科学院农业科技创新跨越工程项目(CX23GG19) （CX23GG19）
黑龙江省重点研发计划项目(GA23B011). （GA23B011）
