中国南方果树2025，Vol.54Issue(5)：21-28,8.DOI:10.13938/j.issn.1007-1431.20240479
低温胁迫下不同澳洲坚果品种的生理响应及抗寒性评价
Physiological response and evaluation of chilling resistance of six macadamia varieties under low temperature stress
摘要
Abstract
In order to analyze the physiological characteristics and chilling resistance of macadamia in response to low-tempera-ture stress,the leaves of six macadamia varieties were used as test materials.Ten physiological indexes,including relative water content,relative conductivity,and the content of soluble sugars,soluble proteins,proline and malondialdehyde(MDA),and the activity of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD)and polyphenol oxidase(PPO)of macadamia leav-es were measured and analyzed through low-temperature stress(-4 ℃)treatment(0 h,24 h,48 h,72 h treatments).The chill-ing resistance was comprehensively evaluated by using correlation analysis,principal component analysis and affiliation function method.The results showed that with the extension of low-temperature stress,the relative conductivity of macadamia leaves in-creased,while the relative water content decreased,the content of proline,soluble proteins,soluble sugars,MDA and the activi-ties of SOD and CAT increased and then decreased,and the overall activities of POD and PPO showed a decreasing and then in-creasing trend.Principal component analysis indicated that eight physiological indexes,namely,MDA,relative water content,relative conductivity,soluble sugar,POD,CAT,soluble protein and proline,could be used as the main indexes for evaluating the chilling resistance of macadamia leaves.The strength of chilling hardiness of 6 macadamia varieties comprehensively evaluated by applying the affiliation function was as following:Nanya No.116＞NanyaNo.3＞Nanya No.12＞A16＞Nanya No.1＞A4.The results of this study can lay a foundation for exploring the mechanism of low-temperature stress response regulation in maca-damia,and selecting and breeding new chilling-resistant macadamia varieties.关键词
澳洲坚果/低温胁迫/生理响应/抗寒性/综合评价Key words
macadamia/low temperature stress/physiological response/chilling resistance/comprehensive evaluation引用本文复制引用
康专苗,耿建建,王代谷,郭广正,何凤平,宋喜梅,涂行浩,曾辉,王文林,陶亮..低温胁迫下不同澳洲坚果品种的生理响应及抗寒性评价[J].中国南方果树,2025,54(5):21-28,8.基金项目
贵州省林木种苗培育补助项目(2025-ZM-19 （）
2024-ZM-39) （）
贵州省林业科研项目(黔林科合[2020]03号) （黔林科合[2020]03号）
农业农村部热带果树生物学重点实验室开放基金 （）
贵州山地澳洲坚果育种长期科研基地(2020132540) （2020132540）
望谟县油桐国家林木种质资源库补助项目资助. （）
