中国奶牛Issue(10)：29-32,4.DOI:10.19305/j.cnki.11-3009/s.2025.10.006
牛源副乳房链球菌Spu-HLJ01的分离鉴定及其致病性研究
Isolation,Identification and Pathogenicity Study of Bovine Streptococcus parauberis Strain Spu-HLJ01
摘要
Abstract
To identify the pathogen causing the respiratory disease outbreak in newly purchased Simmental beef cattle at a farm in Heilongjiang Province,necropsy and aseptic collection of pathological materials were performed on one deceased cow for pathogen isolation and identification.Conventional bacterial isolation and culture methods were used,resulting in the isolation of a β-hemolytic,Gram-positive,chain-forming coccus from the lung tissue.Further identification of the isolated strain was conducted using biochemical tests and 16S rRNA gene sequencing analysis.The results showed that the physicochemical characteristics of the isolated strain were consistent with those of the Streptococcus genus,and its 16S rRNA sequence exhibited the highest homology(98.59%)with nine strains of Streptococcus parauberis.Pathogenicity tests in mice demonstrated that the isolated strain was lethal to the animals.Antimicrobial susceptibility testing revealed that the strain was resistant to commonly used antibiotics,including streptomycin,spectinomycin,kanamycin,oxytetracycline,ceftiofur sodium,tylosin and lincomycin.These findings indicate that the isolated strain is Streptococcus parauberis,marking the first time this bacterium has been isolated from a case of bovine pneumonia in China.This discovery highlights the complexity of pathogens associated with bovine respiratory diseases.关键词
副乳房链球菌/分离与鉴定/系统进化分析/药敏试验/动物致病性试验Key words
Streptococcus parauberis/Isolation and identification/Phylogenetic analysis/Antimicrobial susceptibility testing/Animal pathogenicity testing分类
农业科技引用本文复制引用
妥云晨,周玉龙,金振华,钟一鸣,高萌萌,史佳萱,张哲,廖献茂,王一斌,高维明..牛源副乳房链球菌Spu-HLJ01的分离鉴定及其致病性研究[J].中国奶牛,2025,(10):29-32,4.基金项目
黑龙江省"双一流"学科协同创新成果项目(项目编号:LJGXCG2024-F07) （项目编号:LJGXCG2024-F07）
黑龙江省揭榜挂帅项目(项目编号:2023ZXJ02B03) （项目编号:2023ZXJ02B03）
大学生创新创业项目(项目编号:202310223018和202410223122). （项目编号:202310223018和202410223122）
