中国农学通报2025，Vol.41Issue(29)：15-23,9.
室温不同贮藏年限对野生型箭筈豌豆种子萌发及生理特性的影响
Effect of Storage Years at Room Temperature on Seed Germination and Physiological Characteristics of Wild-type Vicia sativa
摘要
Abstract
To elucidate the effects of room-temperature storage duration on seed germination and physiological characteristics in wild-type Vicia sativa L.,this study used seeds stored for 0-2 years to systematically determine dynamic changes in germination parameters,seed coat elemental composition,nutrient contents,antioxidant enzyme activities,and malondialdehyde(MDA)accumulation.The results demonstrated that:(1)the hard seed rate significantly decreased(P<0.05)with increasing storage duration.Both germination percentage(98.52%)and germination potential(97.43%)peaked after 2 years of storage,while seed coat color shifted from gray to reddish-brown.(2)Seed coat calcium(Ca:3911.64 μg/g)and aluminum(Al:18.09 μg/g)contents significantly decreased(P<0.05),whereas potassium(K:5152.93 μg/g),iron(Fe:92.92 μg/g),and manganese(Mn:37.43 μg/g)contents significantly increased.(3)Catalase(CAT)activity first increased then decreased,peaking at 346.76 μmol/g after 1 year.Peroxidase(POD)activity significantly declined(P<0.05)to its lowest(232.00 U/g)after 2 years.Superoxide dismutase(SOD)activity decreased overall,with the highest value(463.03 U/g)at initial storage(0 year).MDA content increased significantly(P<0.05),reaching 9.04 nmol/g at 2 years.(4)Soluble protein(SP)and starch contents initially rose then slightly declined,bottoming at 51.43 mg/g and 60.02 mg/g(respectively)after 2 years.Soluble sugar(SS)content increased steadily,peaking at 8.05 mg/g at 2 years.Lignin content in seed coats showed no significant change(P>0.05).(5)Correlation analysis revealed that germination potential,germination percentage,and MDA content were significantly negatively correlated(P<0.05)with the hard seed rate,and significantly positively correlated(P<0.05)with seed coat color.In conclusion,wild-type Vicia sativa seeds stored at room temperature for 2 years exhibit high vigor and are ideal sowing material.关键词
野生型箭筈豌豆/室温贮藏/种子萌发/酶活性/种皮/丙二醛/硬实Key words
wild-type Vicia sativa/room temperature storage/seed germination/enzyme activity/seed coat/MDA/hard seed分类
农业科技引用本文复制引用
王红林,杜周和,吴子周,左艳春,严旭,袁正才,邓榆川,肖蔹,陈慧,寇晶..室温不同贮藏年限对野生型箭筈豌豆种子萌发及生理特性的影响[J].中国农学通报,2025,41(29):15-23,9.基金项目
南充市推进乡村振兴项目"高蛋白饲用作物优异资源发掘及产业应用"(23XCZX0025) （23XCZX0025）
四川省农业科学院"1+9"揭榜挂帅科技攻关"综合种养核心技术及智能农机装备"(1+9KJGG008) （1+9KJGG008）
第三次全国农作物种质资源普查与收集行动"四川项目组"(111821301354052034) （111821301354052034）
农业种质资源普查"农业种质资源普查项目-牧草"(51000023T000009852064). （51000023T000009852064）
