中国农学通报2025，Vol.41Issue(29)：30-37,8.
基于代谢组学的文山本地蘘荷种质资源评价
Assessment of Local Zingiber mioga Germplasm Resources in Wenshan Based on Metabolomic
摘要
Abstract
Zingiber mioga(Thunb.)Roscoe is a plant resource with food and medicinal value,as well as health-promoting functions.This study aims to investigate the metabolic product differences in flower buds among various Zingiber mioga(Thunb.)Roscoe varieties in the Wenshan Region.In this study,flower buds from two independently selected varieties('Wenhe1','Wenhe2')and one locally preserved variety of Zingiber mioga(Thunb.)Roscoe were used as experimental materials.An extensive targeted metabolomics analysis utilizing high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was conducted to investigate the differences in metabolites and metabolic pathways among the flower buds of Zingiber mioga(Thunb.)Roscoe varieties.The findings indicated that'Wenhe2'had the highest vitamin C content,whereas the landrace variety possessed the highest crude fiber content.In total,758 metabolites were identified in Zingiber mioga(Thunb.)Roscoe buds,including 145 amino acids and derivatives,and 110 flavonoids.Among these metabolites,94 were differentially expressed between'Wenhe1'and'Wenhe 2',65 between'Wenhe1'and the control group,and 87 between'Wenhe2'and the control group.These variations predominantly occurred in amino acids and their derivatives,as well as sugars and their derivatives.Metabolic pathway enrichment analysis revealed that both tested varieties exhibited significant upregulation in pathways related to amino acid synthesis and sinigrin synthesis compared to the control group.Additionally,in the comparison between'Wenhe1'and'Wenhe2',the metabolic pathways significantly upregulated in'Wenhe1'included the pentose phosphate pathway and carbon fixation in photosynthetic organisms,which were strongly associated with photosynthesis.Under identical conditions,significant differences were observed in the metabolite profiles of'Wenhe1'and'Wenhe2'compared to local varieties,especially in metabolites of practical significance,including those involved in amino acid synthesis and glucosinolate synthesis.These results offer valuable insights for the functional breeding of Zingiber mioga(Thunb.)Roscoe and the exploration and utilization of effective metabolomic resources.关键词
蘘荷/花苞/文山地区/代谢组差异/代谢通路差异/维生素C/纤维素/种质资源Key words
Zingiber mioga(Thunb.)Roscoe/flower bud/Wenshan/metabolomic differences/metabolic pathway differences/vitamin C/cellulose/germplasm resources分类
农业科技引用本文复制引用
王灿,徐杰,赵大伟,石瑶,孙宏伟,袁理春,周琼,张云波,周明敏..基于代谢组学的文山本地蘘荷种质资源评价[J].中国农学通报,2025,41(29):30-37,8.基金项目
文山州袁理春专家工作站"蘘荷资源调查与栽培研究示范项目"(No.WS2021-5). （No.WS2021-5）
