中国农学通报2025，Vol.41Issue(30)：8-17,10.
耐盐碱菌株的筛选及其对玉米幼苗促生效果的评估
Screening of Salt-Alkali-Tolerant Bacterial Strains and Evaluation of Their Growth-Promoting Effects on Maize Seedlings
摘要
Abstract
Soil salinization is a global ecological problem restricting agricultural production,and its bioremediation technology has attracted much attention.In this study,salt-tolerant strains were isolated from saline-alkaline soil in Tianjin,and the salt-promoting ability of the strains on maize seedlings was assessed by NaCl medium screening,16S rRNA gene identification,and sand culture test.Three salt-promoting strains(WQ-08,WQ-26,and BH-39)were isolated and identified as Bacillus subtilis,Priestia megaterium,and B.pumilus,respectively.Under 100 mmol/L NaCl salt stress,when strains WQ-08,WQ-26 and BH-39 were respectively added for co-cultivation with maize seedlings,the growth inhibition of maize seedling MC812 was significantly alleviated:the plant height,stem-leaf dry weight and root dry weight in the WQ-08 treatment group increased significantly by 33.32%,37.04%and 36.84%respectively compared with other treatments,while the root length,stem-leaf fresh weight and root fresh weight in the BH-39 treatment group increased significantly by 27.33%,29.35%and 30.55%respectively compared with other treatments.Physiological indexes showed that the antioxidant enzyme activity of maize seedlings in each treatment group decreased significantly.In the strains WQ-08,WQ-26,and BH-39 treatment groups,the SOD activity of maize seedlings decreased by 9.77%,5.60%and 7.71%,the POD activity by 20.44%,22.24%and 25.27%,and the CAT activity by 34.46%,31.27%and 23.28%(P<0.05).The study demonstrated that the three strains possessed both salt tolerance and growth-promoting abilities,which effectively alleviated the salt stress damage to maize seedlings and provided potential microbial resources for crop improvement in saline and alkaline areas.关键词
盐碱化/微生物修复/盐碱地改良/玉米/盐胁迫/耐盐促生/天津市Key words
salinization/microbial remediation/improvement of saline-alkali land/corn/salt stress/salt tolerance and growth promotion/Tianjin分类
农业科技引用本文复制引用
王娜娜,何宗均,董昱辰,路垚,路来风,赵琳娜,沈晓梅..耐盐碱菌株的筛选及其对玉米幼苗促生效果的评估[J].中国农学通报,2025,41(30):8-17,10.基金项目
国家重点研发计划子课题"盐碱地新型土壤改良剂与肥料产品研发"(2021YFD190090501) （2021YFD190090501）
天津市科技计划项目"菌株的耐盐促生机理及其在菌剂研发中的应用研究"(24YDTPJC00420). （24YDTPJC00420）
