中国农学通报2025，Vol.41Issue(30)：52-59,8.
番茄种子内生细菌的分离鉴定及其对种子萌发的影响
Isolation and Identification of Endophytic Bacteria in Tomato Seeds and Their Effect for Seed Germination
摘要
Abstract
Isolation and identification of functional endophytic bacterial strains from tomato seeds to establish a foundation for developing high-efficiency microbial fertilizers.Tomato seeds were used as the experimental material in this study.Endophytic bacteria were isolated and purified from the seeds using the dilution spread plate method.The phylogenetic analysis of strains was done by partial 16S rRNA gene sequences.The promotion characteristics of the strains were assessed via CAS plate assays,inorganic phosphate plate assays,and the Salkowski colorimetric method.Additionally,a seed germination assay was conducted to evaluate the effects of indole acetic acid(IAA)-producing bacterial strains on tomato seed germination.The results of 16S rRNA gene sequence analysis revealed that nine isolated endophytic bacterial strains from tomato seeds belong to two genera:Enterobacter and Bacillus.The plant growth-promoting trait assays revealed that four strains possessed siderophore-producing ability,and three strains exhibited IAA production ability,among which strains B2 and B4 demonstrated higher IAA yields of 88.30 μg/mL and 69.31 μg/mL,respectively.Notably,both strains also exhibited the ability to dissolve inorganic phosphorus.Germination assays further confirmed that the fermented broth of strain B4 significantly promoted tomato seed germination.This study enriches the tomato endophytic bacterial resource library,and the screened B4 strain demonstrates potential as a plant growth-promoting resource for developing multi-functional microbial fertilizers.关键词
内生细菌/番茄种子/分离鉴定/促生特性/吲哚乙酸(IAA)/种子萌发Key words
endophytic bacteria/tomato seeds/isolation and identification/promotion characteristic/indole acetic acid(IAA)/seed germination分类
农业科技引用本文复制引用
高欢,程雯丽,王一茹,王昊阳,陈东博,王洋..番茄种子内生细菌的分离鉴定及其对种子萌发的影响[J].中国农学通报,2025,41(30):52-59,8.基金项目
黑龙江省省属高等学校基本科研业务费科研项目"外源硅缓解番茄盐胁迫机理研究"(2023-KYYWF-1501). （2023-KYYWF-1501）
