中国农学通报2025，Vol.41Issue(30)：70-78,9.
宁夏枸杞根系内皮层发育及质外体屏障研究
Study on Endodermis Development and Apoplast Barrier in Roots of Lycium barbarum L
摘要
Abstract
To investigate the differentiation of the root endodermis and the formation of the apoplast barrier such as Casparian strips and suberin lamella on apoplastic transport pathways in the woody halophyte Lycium barbarum L.(L.barbarum).Using L.barbarum'Ningqi No.1'as experimental material,the development of endodermis and apoplast barrier in the root system of L.barbarum were studied using freehand sectioning combined with fluorescent tracers exploring propidium iodide(PI)and Na+fluorescent probe(CoroNa Green,AM).The results showed that the development of the endodermis structure of L.barbarum can be divided into four developmental phases according to the sequential appearance of Casparian strips and suberin lamella.During the undifferentiated stage,no Casparian strips formation was found in the cell walls,and PI could freely penetrate across both the exodermis and endodermis into the stele.In the first stage,the Casparian strips was observed depositing in endodermal cells and PI could penetrate through exodermis,but was partially blocked its transport at the endodermis,restricting access to the stele.In stage Ⅱ,with well-developed Casparian strips and suberin lamellae,PI penetration to the stele was completely blocked.In stage Ⅲ,extensive root lignification was observed,accompanied by endodermis degeneration and disappearance of Casparian strips.At the same time,the number of cells associated with the endodermis development showed continuous increasing during root growth,and significant growth was observed from the undifferentiated stage to the appearance of Casparian strips(P<0.05).It indicated that the development of the endodermis in the roots of L.barbarum is divided into four stages.Notably,during the primary growth,the formation of Casparian strips was observed in both endodermis and exodermis of L.barbarum roots,which impeded apoplast flow.However,the exodermis Casparian strips were found lacking apoplastic barrier function.关键词
宁夏枸杞/根解剖结构/内皮层/质外体屏障/凯氏带/木栓层Key words
Lycium barbarum L./root anatomical structure/endodermis/apoplast barrier/Casparian strips/suberin lamella分类
农业科技引用本文复制引用
孟利芳,赵王利,金敏,毛桂莲..宁夏枸杞根系内皮层发育及质外体屏障研究[J].中国农学通报,2025,41(30):70-78,9.基金项目
宁夏自然科学基金"LbVHAd2-LbVHAa3共调控盐胁迫下宁夏枸杞体内离子平衡的分子机制研究"(2024AAC03092) （2024AAC03092）
国家自然科学基金"液泡膜H+-ATPase亚基在宁夏枸杞钠转运调控中的功能解析"(32260408). （32260408）
