中国农学通报2025，Vol.41Issue(30)：79-89,11.
盐碱胁迫对灰漠土氨氧化微生物丰度和群落多样性的影响
Effects of Saline and Alkaline Stress on Abundance and Diversity of Community of Ammonia Oxidizing Microorganisms in Gray Desert Soil
摘要
Abstract
Saline-alkali soils are prevalent in the arid regions of Xinjiang,unique physical and chemical properties lead to low soil fertility and the base ions interfere with nitrogen transformation processes.Currently,the effects of the saline-alkali environment on key nitrifying microorganisms,such as ammonia-oxidizing bacteria,remain poorly understood,limiting research on nitrogen cycling in these soils and hindering efforts to improve saline-alkali land.This study aimed to evaluate the effects of two types of saline-alkali stress(NaCl and Na2CO3+NaHCO3)on the abundance,diversity,and community structure of ammonia-oxidizing microorganisms in gray desert soil from arid areas.This experiment used three treatments,including chloride stress(CS),alkaline stress(AS)and a control group(CK)with no salt-alkali stress,respectively,to evaluate the effects of saline-alkaline stress on cotton growth,soil physicochemical and the abundance and community structure of soil AOA and AOB.The results showed that saline-alkaline stress significantly reduced plant height and dry matter weight of cotton as well as soil NO3-N content and potential nitrification rate(PNR).Soil moisture content,salinity,pH and NH4-N content increased significantly under stress conditions.Both salt and alkaline stress decreased the amoA gene copy numbers of AOA,AOB,and the AOA/AOB ratios,respectively,with AOB showing higher amoA gene copy numbers than that of AOA.PNR was positively correlated with AOA and AOB amoA gene copy numbers.Additionally,the operational taxonomic units(OTUs)of AOB were more abundant than those of AOA under saline alkaline stress with alkaline stress causing a significant decrease in OTUs of AOB.Salt stress significantly decreased the Simpson and Shannon diversity index of AOB,whereas alkaline stress significantly increased the Simpson index of AOB.Salt stress also decreased the Pielou_e index of both AOA and AOB,while,alkaline stress increased the Chao1 index of AOA and AOB.The dominant phylum of the AOA community was Thaumarchaeota,while,Proteobacteria was the dominant phylum in the AOB community.The dominant genera for AOA were Candidatus Nitrosocaldus and Nitrososphaera,and they were Nitrosospira and Nitrosomonas for AOB.Both salt and alkaline stress significantly increased the relative abundance of Candidatus Nitrosocosmicus,Nitrosomonas,while decreasing Nitrososphaera,Nitrosospira.Redundancy analysis showed that soil properties,including SWC and pH played an important role in shaping the AOA and denitrifier communities,while AOB community structure was only significantly correlated with pH.These findings suggest that AOA and AOB contribute to nitrification in alluvial gray desert soils,with pH being the dominant factor affecting the growth of ammonia-oxidizing microorganisms and community structure.关键词
盐碱胁迫/氨氧化古菌/氨氧化细菌/潜在硝化势/高通量测序Key words
saline-alkaline stress/AOA/AOB/potential nitrification rate/high-throughput sequencing分类
农业科技引用本文复制引用
向贵琴,叶扬,张楠,郭慧娟..盐碱胁迫对灰漠土氨氧化微生物丰度和群落多样性的影响[J].中国农学通报,2025,41(30):79-89,11.基金项目
新疆生产建设兵团科技人才类青年科学基金项目"盐碱胁迫对土壤硝化反硝化过程的影响机制和调控途径"(2024DB020). （2024DB020）
