中国农学通报2025，Vol.41Issue(31)：41-46,6.
兰州百合组培增殖系数与鳞茎膨大的关键影响因子研究
Study on Key Factors Influencing the Proliferation Coefficient and Bulb Enlargement in Tissue Culture of Lilium davidii var.unicolor
摘要
Abstract
To address the industrialization bottlenecks of traditional lily propagation,namely the long cycle and low multiplication coefficient,and to optimize its rapid propagation system via tissue culture,this study utilized scale segments from virus-free test-tube plantlets of Lilium davidii var.unicolor as explants.Through single-factor gradient experiments,the effects of hormone combinations(ZT,IAA,KT),sucrose concentration,photoperiod,and culture temperature on the proliferation coefficient of lily scale segments were investigated.Additionally,the regulatory effects of sucrose concentration and hormone combinations on the bulking of test-tube bulbs were analyzed.The results showed that:(1)the optimal conditions for scale segment proliferation were 1.0 mg/L KT+0.5 mg/L ZT+0.2 mg/L IAA(hormone combination),30 g/L sucrose,a culture temperature of 23℃,and a 12 h/d photoperiod.Under these conditions,the maximum proliferation coefficient reached 43,with robust plant growth and a moderate number of leaves,balancing efficiency and cost control.(2)When using a single cytokinin(KT or ZT)alone,1.5 mg/L ZT+0.2 mg/L IAA(proliferation coefficient 29)and 1.5 mg/L KT+0.2 mg/L IAA(proliferation coefficient 27)yielded the best results,but these were significantly lower than the synergistic treatment involving both cytokinins.(3)The optimal combination for test-tube bulb bulking was 0.5 mg/L KT+0.3 mg/L IAA(hormone combination)with 60 g/L sucrose,achieving a bulb formation rate of 96%and a maximum bulking multiple of 3.3.The bulbs exhibited good compactness and significant fresh weight accumulation.Although a higher sucrose concentration(≥90 g/L)could increase the bulking multiple(3.71),it led to a decreased bulb formation rate(89.7%)and abnormal growth.This study clarifies the key regulatory factors and optimal parameters for the proliferation and bulb bulking of Lilium davidii var.unicolor in tissue culture,providing a scientific basis and technical support for the large-scale propagation of its virus-free improved varieties.关键词
兰州百合/组织培养/增殖系数/蔗糖浓度/植物激素/光照时间/培养温度Key words
Lilium davidii var.unicolor/tissue culture/multiplication coefficient/sucrose concentration/phytohormone/radiation time/culture temperature分类
农业科技引用本文复制引用
张鑫,郭志鸿,柴娟,马荣,周锋,王琼,姚卓男..兰州百合组培增殖系数与鳞茎膨大的关键影响因子研究[J].中国农学通报,2025,41(31):41-46,6.基金项目
国有资本经营预算支持省属企业科研项目"兰州百合优良新品种高效选育及脱毒良种规模化繁育技术集成与示范"(2024GZ011) （2024GZ011）
兰州百合优质种源开发关键技术"揭榜挂帅"项目"兰州百合优质种源开发关键技术'揭榜挂帅'项目(第一批)"(2024-5-1) （第一批）
甘肃省科技计划项目"兰州百合种质高效利用和优良新品种快速选育"(22YF7NA028) （22YF7NA028）
甘肃省科技计划项目"兰州百合全产业链高效开发关键技术研究与应用"(24ZDNA006). （24ZDNA006）
