中国农业科技导报2025，Vol.27Issue(11)：83-92,10.DOI:10.13304/j.nykjdb.2024.0333
水稻CesA/Csl基因家族的全基因组鉴定及生物信息学分析
Genome-wide Identification and Bioinformatics Analysis of CesA/Csl Gene Family in Rice
摘要
Abstract
Rice cellulose synthase supergene family is composed of CesA(cellulose synthase)and Csl(cellulose synthase-like protein).In order to understand the sequence characteristics and expression characteristics of the members of the family,and explore their functions,bioinformatics methods were used to identify OsCesA/Csl genes in the whole rice genome.Their physical and chemical properties,conserved motifs,gene structure,systematic evolution,cis-acting elements,chromosome mapping and collinearity were analyzed.Their expression in different tissues and different plant hormone treatments were analyzed based on transcriptome data.The results showed that a total of 22 members of OsCesA/Csl family were identified in rice genome,and the length of gene coding sequence(CDS)was 774～3 648 bp,protein molecular weight was 28.55～132.16 kD,isoelectric point was 6.09～9.62,and average hydrophilic coefficient was-0.339～0.750.The subcellular localization of 22 members was on cell membrane or Golgi apparatus.Phylogenetic evolution divided 22 members into 2 categories:OsCesA and OsCsl,each containing 11 members.The family members were distributed on 11 chromosomes except chromosome 11.There were 2 sets of tandem repeat genes in rice,and there was better collinearity between rice and maize.OsCesA/Csl members had different tissue expression patterns,and each member responded to jasmonic acid,abscisic acid,gibberellin and auxin treatment in different degrees.Above results laid a foundation for further exploring the specific function of OsCesA/Csl gene family in the growth and development of rice.关键词
水稻/纤维素合成酶/全基因组鉴定/生物信息学Key words
rice/cellulose synthases/genome-wide identification/bioinformatics分类
农业科学引用本文复制引用
吴荣菊,陈能刚,李欢,鄢小青,杨占烈,刘军..水稻CesA/Csl基因家族的全基因组鉴定及生物信息学分析[J].中国农业科技导报,2025,27(11):83-92,10.基金项目
贵州省自然科学基金项目(黔科合基础-ZK[2022]一般243) （黔科合基础-ZK[2022]一般243）
贵州省农业科学院青年基金项目(黔农科一般基金[2024]06). （黔农科一般基金[2024]06）
