中国农业科技导报2025，Vol.27Issue(11)：102-110,9.DOI:10.13304/j.nykjdb.2024.1034
陆地棉苗期耐低氮种质鉴评与筛选
Evaluation and Screening on Nitrogen Deficiency Tolerant Germplasm of Upland Cotton at Seedling Stage
摘要
Abstract
In order to analyze the nitrogen deficiency tolerance of cotton germplasm resources,in pots with low nitrogen(15 g·m-2)and normal nitrogen(45 g·m-2)conditions under a rainproof shelter at the seedling stage,root dry weight,shoot dry weight,total dry weight,ratio of root to shoot and chlorophyll value of 419 upland cotton germplasm resources were measured,and their nitrogen deficiency tolerance indexes were calculated.Through correlation analysis,principal component analysis and membership function analysis,the comprehensive evaluation of nitrogen deficiency tolerance of 419 cotton germplasm resources was conducted.The results showed that,under low nitrogen stress,there was extremely significantly negative correlation between the nitrogen resistance index in shoot dry weight and in ratio of root to shoot of cotton seedlings.However,other traits showed significantly positive correlations or extremely significantly positive correlations.Principal component analysis classified 5 indexes into 3 principal components,whose cumulative variance contribution rate was 98.590%.D-values were calculated based on the weights of these 3 principal components.Then the 419 germplasms were divided into 4 grades and 10 nitrogen deficiency tolerant germplasms were screened.Furthermore,through stepwise regression analysis,a regression equation was established as D=-0.123+0.167XRDWNRI+0.252XTDWNRI+0.132XSPADNRI(XRDWNRI,XTDWNRI and XSPADNRI were nitrogen resistance indexes of root dry weight,total dry weight and chlorophyll value).Above results laid foundation for further mining of nitrogen deficiency tolerant genes and improving nitrogen deficiency tolerance of cotton.关键词
陆地棉/核心种质/苗期/耐低氮/筛选Key words
upland cotton/core collection/seedling stage/nitrogen deficiency tolerance/screening分类
农业科学引用本文复制引用
吕星,王星懿,孙正文,柯会锋,王国宁,张艳,王省芬,马峙英,谷淇深..陆地棉苗期耐低氮种质鉴评与筛选[J].中国农业科技导报,2025,27(11):102-110,9.基金项目
河北省自然科学基金项目(C2022204205) （C2022204205）
河北省高端人才支持计划项目(031601801). （031601801）
