中国农业科技导报2025，Vol.27Issue(11)：164-173,10.DOI:10.13304/j.nykjdb.2025.0529
枣植原体分泌蛋白ncSP35抑制超敏反应及植物生长发育
A Non-classical Secretory Protein ncSP35 of Candidatus Phytoplasma Ziziphi Inhibits Hypersensitive Response and Plant Growth and Development
摘要
Abstract
Jujube witches'broom(JWB),caused by Candidatus Phytoplasma ziziphi,is currently the most devastating jujube disease.A novel non-classical secretory protein,ncSP35,was identified from the JWB phytoplasma through bioinformatics analysis combined with an Escherichia coli-based alkaline phosphatase reporter system.Transient expression of ncSP35 in Nicotiana benthamiana using a potato virus X-based expression vector demonstrated that the protein suppressed Bax-triggered hypersensitive response(HR)and hydrogen peroxide accumulation,and interfered with plant growth and development.Deletion and site-directed mutagenesis analyses revealed that the central region(amino acid residues 37～71)and the polar amino acid cluster HKKENK⁸² of ncSP35 were critical for HR suppression,while the C-terminal 18 amino acid residues were essential for interfering with plant growth and development.Subcellular localization analysis showed that ncSP35 formed abundant aggregates in the cytoplasm and inhibited the nuclear localization of histone H2B,causing H2B to also form aggregates in the cytoplasm.Yeast two-hybrid,co-immunoprecipitation and luciferase complementation assays confirmed the specific interaction between ncSP35 and jujube ZjH2B.Above results provided new molecular insights for JWB pathogenesis.关键词
枣植原体/非典型分泌蛋白/超敏反应/植物生长发育/组蛋白H2B/蛋白互作Key words
candidatus phytoplasma ziziphi/non-classical secretory protein/hypersensitive response/plant growth and development/histone 2B/protein-protein interaction分类
农业科技引用本文复制引用
金玉倩,傅子恒,高小语,李为民..枣植原体分泌蛋白ncSP35抑制超敏反应及植物生长发育[J].中国农业科技导报,2025,27(11):164-173,10.基金项目
国家自然科学基金项目(32170190) （32170190）
北京市自然科学基金项目(6252003). （6252003）
