中国农业科学2025，Vol.58Issue(20)：4117-4130,14.DOI:10.3864/j.issn.0578-1752.2025.20.007
利用水稻耐盐品种Pokkali衍生材料选育的高产耐盐新种质的遗传变异
Genetic Variation Analysis of New Germplasm with Combined High Yield and Salt Tolerance Developed from Derivative Materials of Pokkali
摘要
Abstract
[Objective]This study aimed to elucidate the genetic basis of high yield and salt tolerance in rice through comparative genomic analysis of the salt-tolerant paternal parent Pokkali-D(Pokkali-Dwarf),the high-yielding maternal parent Huazhan,and their derived line Zhongyandao 16.The findings provide a theoretical foundation and genetic resources for the molecular breeding of salt-tolerant rice.[Method]A new germplasm named Zhongyandao 16,which combines salt tolerance and high yield,was developed by crossing the salt-tolerant parent Pokkali-D with the high-yielding parent Huazhan.The salt tolerance of Zhongyandao 16 was evaluated based on seedling survival rate under salt stress(120 mmol·L-1NaCl)and yield performance in a saline field(0.4％salinity).To further investigate the genetic basis of its superior traits,whole-genome resequencing of Pokkali-D,Huazhan and Zhongyandao 16 was conducted.Genetic variants inherited from both parents,as well as unique variants present in Zhongyandao 16,were screened through variant detection.High-impact variants(causing frameshifts or premature termination of translation)and moderate-impact variants(resulting in amino acid changes)were annotated for the affected genes.[Result]Under 120 mmol·L-1 NaCl stress,the seedling survival rate of Zhongyandao 16 was similar to that of Pokkali-D and significantly higher than that of Huazhan.Under normal field conditions,the yield of Zhongyandao 16 was comparable to Huazhan and significantly higher than Pokkali-D.Under 0.4％salinity field conditions,Zhongyandao 16 exhibited significantly higher yield than both parents,demonstrating a combination of salt tolerance and high yield.Comparative genomic analysis against the Nipponbare reference genome showed that Zhongyandao 16 shared 86 716 identical variants with Huazhan,among which high-and moderate-impact variants were annotated to 1 629 genes.It also shared 21 623 identical variants with Pokkali-D,with high-and moderate-impact variants annotated to 443 genes.Additionally,Zhongyandao 16 carried 372 unique variants,with high-and moderate-impact variants annotated to 11 genes.Functional screening of these 2 083 genes identified 16 involved in salt stress regulation(12 derived from Huazhan,4 from Pokkali-D),48 associated with yield-related traits(36 from Huazhan,12 from Pokkali-D),and 3 modulated both yield and salt tolerance(2 from Huazhan,1 from Pokkali-D).Notably,salt-tolerance alleles from Pokkali-D including OsPRPL18,OsSTLK,STH1,OsLPR2,and OsLPR5,as well as those from Huazhan,such as OsSAP4,OsY3IP1,OsABI5 and OsHKT1;5,were pyramided in Zhongyandao 16.[Conclusion]By pyramiding yield-related genes from Huazhan and salt-tolerance alleles from Pokkali-D,Zhongyandao 16 combines high yield and salt tolerance.This demonstrates that introducing key salt-tolerant alleles from Pokkali-D into the high-yielding Huazhan background can effectively enhance salt tolerance without compromising yield.关键词
水稻/新种质/耐盐性/高产/变异位点/基因聚合Key words
rice/new germplasm/salt tolerance/high yield/genetic variants/gene pyramiding引用本文复制引用
刘春蕾,王娟..利用水稻耐盐品种Pokkali衍生材料选育的高产耐盐新种质的遗传变异[J].中国农业科学,2025,58(20):4117-4130,14.基金项目
国家自然科学基金(32171949) （32171949）
