中国农业科学2025，Vol.58Issue(20)：4144-4157,14.DOI:10.3864/j.issn.0578-1752.2025.20.009
西藏拉鲁湿地野生植物根际微生物资源挖掘及其在缓解玉米盐碱胁迫中的作用
Characterization and Utilization of Rhizosphere Microbiota from Wild Plants in Lalu Wetland in Xizang for Alleviating Saline-Alkali Stress in Maize
摘要
Abstract
[Objective]Soil salinization poses a significant constraint on global agricultural development.However,microorganisms have shown the potential to mitigate salt stress in plants,and long-term intensive cultivation often leads to the unbalance of the beneficial microbial communities in agricultural soils.Isolating plant growth-promoting bacteria from the rhizosphere of wild plants in less disturbed habitats and introducing them into saline-alkali farmland offers a promising strategy to enhance crop tolerance to salt-alkali stress.[Method]Isolation and culture of rhizospheric bacteria were performed using samples from five wetland plant species collected in the Lalu Wetland National Nature Reserve,Tibet.The isolates were preliminarily identified based on 16S rRNA gene sequencing.Functional characterization was further carried out using specific identification media.Finally,pot experiments were conducted to assess the effects of the selected promising microbial strains on the growth of maize seedlings.[Result]A total of 260 strains were isolated from the rhizosphere soil of Erigeron canadensis,Galinsoga parviflora,Datura stramonium,Taraxacum mongolicum and Astragalus membranaceus,including 14 species and 21 potential novel species,and the dominant genus was Acinetobacter.34 strains exhibiting inorganic phosphate-solubilizing capabilities and 47 strains demonstrating siderophore-producing functions were isolated through screening.Among them,strains 3-210 and 3-218 were identified as potential novel species of the genus Flavobacterium through genome analysis,and one key gene for plant hormone signal transduction GS2 was predicted.FeGenie predicted 50 and 71 iron related genes,respectively.No virulence factor genes related to the pathogenic process were detected.At the same time,the results of the pot experiment showed that inoculation of these two strains could improve the agronomic traits,such as plant height and stem diameter of maize seedlings.3-210 treatment significantly increased the catalase activity and free proline content in leaves,and 3-218 group had significantly higher activities of the catalase and peroxide.[Conclusion]The rhizospheric soils of plants in the Lalu Wetland National Nature Reserve,Tibet,harbored abundant functional bacterial resources,including multiple strains capable of siderophore production and phosphate solubilization.Notably,two potential novel species of the genus Flavobacterium demonstrated the ability to enhance saline-alkali stress tolerance in maize seedlings.关键词
拉鲁湿地国家级自然保护区/盐碱地/PGPR/玉米/黄杆菌属Key words
Lalu Wetland National Nature Reserve/saline-alkali land/plant growth promoting rhizobacteria/maize/Flavobacterium引用本文复制引用
杨芾,韦善君,张晓霞,张琳敏,刘洪秀,李怡君,仝亚萍..西藏拉鲁湿地野生植物根际微生物资源挖掘及其在缓解玉米盐碱胁迫中的作用[J].中国农业科学,2025,58(20):4144-4157,14.基金项目
中国农业科学院科技创新工程(CAAS-ZDRW202407) （CAAS-ZDRW202407）
