中国生物防治学报2025，Vol.41Issue(5)：1063-1077,15.DOI:10.16409/j.cnki.2095-039x.2024.11.008
CO2浓度增加条件下球孢白僵菌定殖对玉米促生和抗性作用的转录组学分析
Transcriptomic Analysis of the Effects of Beauveria bassiana Colonization on Maize Growth and Resistance under Elevated CO2
摘要
Abstract
The objective of this study was to clarify the underlying molecular mechanism of Beauveria bassiana colonization on plant growth and resistance under elevated CO2.The study,employing maize B73 cultivar as the plant material and open top chambers for enrichment of CO2,measured the growth and stress resistance of maize plants with B.bassiana colonization and explored the underlying mechanism through transcriptome sequencing and bioinformatics analysis.Transcriptome sequencing and fluorescence quantitative PCR confirmed that,when compared with normal atmospheric CO2 concentration(Control),the increase of CO2 concentration(eCO2)and the colonization of B.bassiana(Bb)had significant effects on the expression of genes related to resistance and growth promotion in maize.The comparisons of Bb vs eCO2+Bb,Control vs Bb,Control vs eCO2,and eCO2 vs eCO2+Bb revealed 907,803,589,and 669 differentially expressed genes(DEGs),respectively,which included 407,429,360,and 238 genes with up-regulated expression and 500,374,229,and 431 genes with down-regulated expression,respectively.Among these DEGs,16 were common in the pairwise comparisons of samples from different treatment groups.The DEGs related to plant growth and resistance were mainly enriched in metabolic pathways,biosynthesis of secondary metabolites,glycosaminoglycan degradation,steroid biosynthesis,the pathways of diterpenoid biosynthesis and benzoxazinoid biosynthesis.The qRT-PCR verified the transcriptome analysis results of DEGs.The increase of CO2 concentration and the colonization of B.bassiana have a positive regulatory effect on the functional genes and pathways related to maize growth and resistance to a certain extent,and then have a positive impact on the growth and development of maize plants.关键词
气候变化/虫生真菌/内生定殖/促生作用/转录组Key words
climate change/entomopathogenic fungi/endophytic/growth promotion/transcriptome分类
植物保护学引用本文复制引用
文欢,张正坤,路杨,隋丽,李启云..CO2浓度增加条件下球孢白僵菌定殖对玉米促生和抗性作用的转录组学分析[J].中国生物防治学报,2025,41(5):1063-1077,15.基金项目
国家自然科学基金(32271683) （32271683）
吉林省农业科技创新工程(CXGC2024JJ011) （CXGC2024JJ011）
