中国生物防治学报2025，Vol.41Issue(5)：1179-1187,9.DOI:10.16409/j.cnki.2095-039x.2025.02.058
草莓根腐病病原菌的分离鉴定及哈茨木霉TW21990对其防治效果
Isolation and Identification of Trichoderma harzianum TW21990 and Its Control Effect on Strawberry Root Rot
摘要
Abstract
The aim of this study was to screen antagonistic Trichoderma strains with biocontrol effects against strawberry root rot in Licheng District,Jinan city.The pathogens causing strawberry root rot were identified based on morphology and ITS sequence.The antagonistic Trichoderma strains were screened against dominant pathogens with strong susceptibility,and the biocontrol effect of selected Trichoderma strains was evaluated by pot experiment and field test.The results showed that the dominant pathogens of strawberry root rot in Licheng District were Colletotrichum spp.,among which a highly susceptible strain of CM9 was identified as C.siamense by polygene sequence characterization.Trichoderma harzianum TW21990 was selected for its excellent antagonistic activity by plate confrontation,with inhibition rates of volatile and soluble metabolites against C.siamense CM9 reaching 53.32％and 63.24％,respectively.Pot experiment and field test demonstrated that control efficacy of TW21990 against strawberry root rot was 61.14％and 60.70％,respectively.In addition TW21990 treatment significantly improved the quality of strawberry fruit.The content of vitamin C,soluble sugar and soluble solids in the fruit with increased by 32.89％,32.06％and 11.96％respectively compared with the control group.In conclusion,TW21990 showed great application potential for strawberry production.关键词
草莓根腐病/病原菌鉴定/哈茨木霉TW21990Key words
strawberry root rot/pathogen identification/Trichoderma harzianum TW21990分类
植物保护学引用本文复制引用
李红梅,魏艳丽,杨凯,扈进冬,李杨,赵忠娟,李纪顺,杨建闻..草莓根腐病病原菌的分离鉴定及哈茨木霉TW21990对其防治效果[J].中国生物防治学报,2025,41(5):1179-1187,9.基金项目
齐鲁工业大学(山东省科学院)科教产融合试点工程重大创新类项目(2025ZDZX18),齐鲁工业大学(山东省科学院)科教产融合创新试点工程国合专项项目(2024GH21、2024GH15) （山东省科学院）
山东省科技型中小企业创新能力提升工程项目(2022TSGC2370) （2022TSGC2370）
枣庄市国家可持续发展议程创新示范区科技专项(2024SFQZX007) （2024SFQZX007）
