中国生物防治学报2025，Vol.41Issue(5)：1200-1216,17.DOI:10.16409/j.cnki.2095-039x.2025.02.063
多粘类芽胞杆菌NK-1的鉴定、发酵优化及其对采后马铃薯干腐病的生防效果研究
Paenibacillus polymyxa NK-1:Identification,Fermentation Optimization,and Biocontrol Effect on Dry Rot Disease of Postharvest Potato
摘要
Abstract
In order to obtain strains with potential biocontrol ability against potato dry rot,NK-1,a strain with remarkable antimicrobial activity against Fusarium spp.,was isolated using the plate confrontation assay in the present study.Strain NK-1 demonstrated antagonism against five pathogens,with inhibition rates of 47.31％-91.08％,and produced amylase,protease,and cellulase.Combined with morphological observations,physiological and biochemical characteristics,and multi-gene phylogenetic tree analysis,NK-1 was identified as Paenibacillus polymyxa.Through single-factor and orthogonal tests,the optimal fermentation components and conditions for NK-1 were determined as:1％sucrose,7％beef extract,0.1％NaCl,0.05％MgSO4,temperature 28℃,agitation speed 180 r/min,fermentation time 48 h,culture volume 50 mL(in 250 mL conical flasks),inoculum concentration 2.5％,and initial pH 8.Under these conditions,the biomass of NK-1 was approximately 60 times more than that obtained under pre-optimisation conditions.Additionally,its antimicrobial activity growth rate ranged from 4.16％—17.23％.Moreover,the fermentation broth of NK-1 could induce deformation and disintegration of pathogenic hyphae.The germination rate of pathogenic fungal spores treated with 108 CFU/mL of NK-1 fermentation broth ranged from 16.17％—24.50％.Results of the potato storage experiment showed that the NK-1 fermentation broth exhibited significant efficacy in controlling potato dry rot.The preventive and therapeutic effects were 30.10％and 23.01％,respectively,by day 30.This demonstrates that strain NK-1 possesses promising biocontrol potential against potato post-harvest dry rot,providing a new microbial resource for biological control of Fusarium dry rot.关键词
马铃薯干腐病/多粘类芽胞杆菌/优化/贮藏/生防效果Key words
potato dry rot/Paenibacillus polymyxa/optimization/storage/biocontrol effect分类
农业科学引用本文复制引用
郭家如,师玉沛,赵迎丽,王亮..多粘类芽胞杆菌NK-1的鉴定、发酵优化及其对采后马铃薯干腐病的生防效果研究[J].中国生物防治学报,2025,41(5):1200-1216,17.基金项目
马铃薯提质增效关键技术研发与应用(2024XDHZ05) （2024XDHZ05）
山西省薯类产业技术体系建设项目(2025CYJSTX06-18) （2025CYJSTX06-18）
山西省农业科学院科技创新研究课题(YCC2020202) （YCC2020202）
山西省重点研发计划项目(201903D221039) （201903D221039）
山西省科技合作交流专项项目(202304041101054) （202304041101054）
