中国生物防治学报2025，Vol.41Issue(5)：1217-1230,14.DOI:10.16409/j.cnki.2095-039x.2025.02.062
响应面法优化发酵培养基提高Xenorhabdus budapestensis XBD8菌株的GameXPeptide-A产量
Optimization of Fermentation Media via Response Surface Methodology Enhances GameXPeptide-A Yield in Xenorhabdus budapestensis XBD8
摘要
Abstract
GameXPeptide-A(GXP-A),a cyclic peptide natural product,exhibits significant inhibitory activity against the innate immune systems of agricultural pests,making it a highly promising candidate for pest control.In this study,we systematically optimized the fermentation medium for Xenorhabdus budapestensis XBD8,an entomopathogenic nematode symbiotic bacterium isolated by our team,to boost GXP-A production and facilitated its large-scale application.Initially,single-factor and combinatorial experiments were used to identify the optimal carbon sources,nitrogen sources,inorganic salts,and their concentrations in the culture medium.The Plackett-Burman design was then employed to screen the key factors influencing bacterial growth,after which the steepest ascent experiment was used to approach the response surface center.Finally,a regression model was established based on the Box-Behnken design,which allowed us to determine the optimal fermentation medium composition:yeast extract 25.77 g/L,ferric chloride 0.30 g/L,sodium chloride 5.49 g/L,tryptone 10.0 g/L,magnesium sulfate 2.0 g/L,and beef extract 14.0 g/L.The optimized medium significantly enhanced GXP-A production.In 250-mL shake flask experiments,GXP-Ayields reached 8.92 mg/L,and in a 5-L bioreactor setup,yields further increased to 20.87 mg/L.Compared to pre-optimization yields,this represents remarkable increases of 869.57％and 2168.48％,respectively.关键词
布达佩斯致病杆菌XBD8/GameXPeptide-A/响应面法/发酵培养基优化Key words
Xenorhabdus budapestensis XBD8/GameXPeptide-A/response surface analysis/fermentation optimization分类
植物保护学引用本文复制引用
李鑫瑶,贾丰莲,王毓琦,涂雄兵,李广悦..响应面法优化发酵培养基提高Xenorhabdus budapestensis XBD8菌株的GameXPeptide-A产量[J].中国生物防治学报,2025,41(5):1217-1230,14.基金项目
重点研发计划项目(2022YFD1400500) （2022YFD1400500）
