中国输血杂志2025，Vol.38Issue(10)：1307-1312,6.DOI:10.13303/j.cjbt.issn.1004-549x.2025.10.002
CaMKⅡδ在凝血酶及凝血酶受体激活肽介导的血小板活化中的作用
The role of CaMKⅡδ in platelet activation mediated by thrombin and TRAP
摘要
Abstract
Objective To investigate the role of calcium/calmodulin-dependent protein kinase Ⅱ delta(CaMKⅡδ)in platelet activation mediated by thrombin and thrombin receptor activator peptide(TRAP).Methods The CaMKⅡδ-specific inhibitor Hesperadin was used to assess its inhibitory effects on platelet activation using multiple detection methods.These included:analyzing the inhibitory effect of different concentrations of Hesperadin on thrombin-and TRAP-induced platelet aggregation using a platelet aggregometer;measuring CD62P expression levels and calcium mobilization via flow cytometry;and determining dense granule release activity using a microplate reader.Results Hesperadin at concentrations of 40 μM and 80 μM significantly inhibited thrombin-and TRAP-induced platelet aggregation(P<0.05).Within the concentration range of 20-80 μM,it significantly inhibited α-granule release and TRAP-induced calcium ion mobilization(P<0.05);at 80 μM,it markedly suppressed thrombin-induced platelet calcium ion mobilization and significantly inhibited dense gran-ule release(P<0.05).Conclusion The experimental results indicate that CaMKⅡδ in platelets is activated and plays a regulatory role in the process of platelet activation induced by thrombin and TRAP.关键词
血小板/钙信号/止血/PAR受体/CaMKⅡδ/HesperadinKey words
platelets/calcium signaling/hemostasis/PAR receptors/CaMKⅡδ/Hesperadin分类
临床医学引用本文复制引用
赵娅菲,陈永莹,孙盼,蒋鹏,马莉..CaMKⅡδ在凝血酶及凝血酶受体激活肽介导的血小板活化中的作用[J].中国输血杂志,2025,38(10):1307-1312,6.基金项目
国家自然科学基金(31801192) （31801192）
