中国糖料2025，Vol.47Issue(4)：1-10,10.DOI:10.13570/j.cnki.scc.2025.04.001
甜菜BvMT2基因的生物信息学及镉应答特性分析
Bioinformatics and Cadmium Response Characteristics Analysis of Sugar Beet BvMT2 Gene
摘要
Abstract
[Objective]Metallothioneins(MT),widely distributed metal-binding proteins in organisms,play crucial roles in plants'resistance to heavy metal stress.This study aimed to investigate the response characteristics of sugar beet metallothionein 2(BvMT2)under cadmium(Cd)stress.[Methods]The gene was cloned using RT-PCR technology,and bioinformatics methods were employed to predict cis-acting elements,upstream transcription factors,and physicochemical properties of the gene.Its transcriptional expression under varying Cd stress concentrations was analyzed.[Results]The coding region of the gene is 225 bp,with a relative molecular weight of 1 909.91 Da.It has a theoretical isoelectric point of 4.55 and is characterized by high contents of cysteine(Cys)and glycine(Gly),classifying it as an unstable,non-secretory,hydrophilic protein likely localized in the nucleus or chloroplasts.BvMT2 contains seven relevant cis-acting elements including CGTCA-motif,auxin related,light-response,partial light-responsive elements with conserved sequences,MYB-binding,anaerobic induction,and regulation.Transcription factors BVRB_7g177640 and BVRB_3g048050 are implicated in the transcriptional regulation of BvMT2.Transcriptome data indicated significant upregulation of BvMT2 under Cd stress at concentrations of 1,3 and 5 mmol/L.[Conclusion]In conclusion,BvMT2 plays a crucial role in sugar beet's response to Cd stress.This study establishes a foundation for understanding the function of BvMT2 in responding to heavy metal stress and provides insights into the mechanisms of sugar beet response to heavy metal pollution.关键词
甜菜/金属硫蛋白(MT)/镉胁迫/生物信息学/转录表达特性Key words
sugar beet/metallothionein(MT)/cadmium(Cd)stress/bioinformatics/transcriptional expression characteristics分类
农业科学引用本文复制引用
黄烁淇,赵桂龙,赵晓鑫,刘大丽,鲁振强..甜菜BvMT2基因的生物信息学及镉应答特性分析[J].中国糖料,2025,47(4):1-10,10.基金项目
国家糖料产业技术体系项目(CARS-17) （CARS-17）
甜菜种质资源安全保存项目(19240442) （19240442）
甜菜种质资源精准鉴定项目(19240700) （19240700）
黑龙江省高校科研业务费项目(2022-KYYWF-1070) （2022-KYYWF-1070）
黑龙江省自然科学基金(LH2023C090) （LH2023C090）
内蒙古自治区"揭榜挂帅"项目(2022JBGS0029)资助. （2022JBGS0029）
