中国现代医学杂志2025，Vol.35Issue(21)：22-29,8.DOI:10.3969/j.issn.1005-8982.2025.21.004
低氧诱导因子-1α对牙周炎氧化应激与铁死亡影响的初步研究
A preliminary investigation into the role of hypoxia-inducible factor-1α in oxidative stress and ferroptosis associated with periodontitis
摘要
Abstract
Objective To investigate the expression level of hypoxia-inducible factor-1α(HIF-1α)in periodontal tissues and its effects on oxidative stress and ferroptosis,while exploring their interrelationships.Methods Forty 6-8-week-old male Sprague-Dawley(SD)rats were randomly divided into four groups:normoxia control(Group A),normoxia periodontitis(Group B),hypoxia control(Group C),and hypoxia periodontitis(Group D).Periodontitis and hypoxia models were established accordingly.At 1,3,and 6 weeks post-modeling,gingival tissues were collected for hematoxylin-eosin(HE)staining to evaluate inflammatory status.Immunohistochemistry(IHC)was performed to detect protein expression levels of HIF-1α,ferritin heavy chain 1(FTH1),and glutathione peroxidase 4(GPX4).Serum levels of reactive oxygen species(ROS),GPX4,and HIF-1α were measured by enzyme-linked immunosorbent assay(ELISA).Results HE staining:Compared with Group A,Group B showed progressively aggravated inflammation,while Group D exhibited significant inflammatory cell infiltration during early and middle stages with attenuated inflammation at the later stage.IHC staining:HIF-1α and FTH1 expression levels were significantly elevated in Groups B,C and D compared with Group A(P<0.05).During early and middle stages,Group D demonstrated higher HIF-1α and FTH1 expression than Group B,while at the later stage Group D showed increased GPX4 expression compared to Group B.ELISA:Group D displayed significantly elevated HIF-1α and ROS levels at early stage(P<0.05).During middle stage,the hypoxia groups exhibited higher GSH expression than Group B(P<0.05),while at the later stage the Group C and Group D showed lower ROS levels than Group A and Group B(P<0.05).Conclusion The HIF-1α activated under hypoxic conditions,is associated with the initiation and progression of periodontal inflammation.Furthermore,HIF-1α may contribute to oxidative stress and ferroptosis in periodontal tissues by regulating reactive oxygen species production and iron metabolism.关键词
牙周炎/低氧诱导因子-1α/氧化应激/活性氧/铁死亡/低氧Key words
periodontitis/hypoxia-inducible factor-1α/oxidative stress/reactive oxygen species/ferroptosis/hypoxia分类
口腔医学引用本文复制引用
王彩雯,罗晓洁,范丽君,张艳君,陈晓涛,是文辉..低氧诱导因子-1α对牙周炎氧化应激与铁死亡影响的初步研究[J].中国现代医学杂志,2025,35(21):22-29,8.基金项目
国家自然科学基金地区项目(No:82260195) （No:82260195）
新疆维吾尔自治区自然科学基金(No:2021D01C159) （No:2021D01C159）
天山英才医药卫生高层次人才培养项目(No:TSYC202301A016) （No:TSYC202301A016）
