中国畜牧兽医2025，Vol.52Issue(11)：5030-5039,10.DOI:10.16431/j.cnki.1671-7236.2025.11.002
转录组测序中核糖体RNA消减策略:研究进展与方法比较
Ribosomal RNA Reduction Strategies in RNA-Seq:Research Progress and Method Comparison
摘要
Abstract
RNA sequencing(RNA-Seq)technology is a powerful tool that reveals various complex biological phenomena by analyzing the transcriptional levels of genes and regulatory elements within cells.It has a wide range of applications in fields such as agricultural breeding,variety resources,nutrition metabolism and medicine.Ribosomal RNA(rRNA)accounts for over 80％of the total RNA in cells.Direct RNA-Seq library sequencing of samples yields data in which more than 90％of the sequences from rRNA,making it difficult to detect various low abundance RNAs.rRNA reduction technology can significantly improve the gene detection efficiency and sensitivity of RNA-Seq technology,and can quantitatively detect low abundance non-coding RNA,providing more accurate data support for revealing some complex biological mechanisms.However,the role of this technology in RNA-Seq library construction has often not been given due attention,resulting in some sequencing data not reflecting the true value of the sample.The authors provide an overview of several rRNA reduction strategies in RNA-Seq,including Olig dT magnetic bead capture method,"pull-out"method,RNase H selective reduction method,and thermostable duplex-specific nuclease(DSN)selective method,non random primer reverse transcription method,and RNA blocking primer methods,etc.The advantages and disadvantages of each strategy are compared,and intended to select the most suitable ribosomal RNA reduction strategy for researchers employing RNA-Seq technology,provide support and reference for obtaining research data that is closest to the true value of the sample,and enable new technologies to better serve scientific research and technological innovation.关键词
转录组测序/核糖体RNA/消减策略Key words
RNA-Seq/rRNA/strategies reduction分类
水产学引用本文复制引用
李慧彤,沈青春,熊涛,刘丹,高建帅,唐新月,张博源,蒋卉,范学政,张广智,丁家波..转录组测序中核糖体RNA消减策略:研究进展与方法比较[J].中国畜牧兽医,2025,52(11):5030-5039,10.基金项目
"十四五"国家重点研发计划项目-重大外来动物疫病阻断与防控技术研发项目(2022YFD1800500) （2022YFD1800500）
