中国畜牧兽医2025，Vol.52Issue(11)：5040-5052,13.DOI:10.16431/j.cnki.1671-7236.2025.11.003
不同运动性能伊犁马80 km耐力赛能量代谢特征差异研究
Study on Difference of Energy Metabolism Characterization in Yili Horses with Different Exercise Performances in an 80 km Endurance Race
摘要
Abstract
[Objective]This study was aimed to explore the difference of plasma energy metabolites in Yili horses with different exercise performances in an 80 km endurance race.[Method]Eighteen Yili horses participating in an 80 km endurance race were selected.According to their race completion status and heart rate recovery ability,the horses were divided into three groups:Excellent group(finished the race with a qualified heart rate),normal group(finished the race but with delayed heart rate recovery),and poor group(did not finish the race),with 6 horses in each group.Cervical venous blood samples were collected at rest before the race and within 10 minutes after the race,and plasma was prepared.Non-targeted metabolomics detection was performed on the plasma samples using liquid chromatography-mass spectrometry(LC-MS)to screen for differential energy metabolites in Yili horses with different exercise performances and conduct pathway enrichment analysis.[Result]The contents of 11 energy differential metabolites such as L-aspartic acid,isocitric acid,DL-3-phenyllactic acid,guanosine diphosphate,cis-aconitic acid and itaconic acid in plasma of Yili horses in excellent group were significantly higher than those in normal and poor groups,while the content of glycolic acid was significantly lower than that in normal and poor groups.The contents of L-aspartic acid and serine in plasma of Yili horses at pre-race in excellent group were significantly up-regulated compared with those at post-race,and the contents of 15 metabolites such as isocitric acid,DL-3-phenyllactic acid,itaconic acid,cis-aconitic acid and ureidopropionic acid were significantly down-regulated.The energy differential metabolites were mainly enriched in the metabolic pathways of pantothenic acid and coenzyme A(CoA)biosynthesis,β-alanine,C5 branched dicarboxylic acid and lysine biosynthesis.[Conclusion]There were significant differences in the contents of energy differential metabolites such as itaconic acid,isocitric acid,DL-3-phenyllactic acid and cis-aconitic acid among different groups and between pre-race and post-race in excellent group,indicating that they were differential metabolites affecting endurance exercise and could be used as potential biomarkers.The energy differential metabolites were mainly enriched in pantothenic acid and CoA biosynthesis metabolic pathway,β-alanine metabolic pathway,C5 branched dicarboxylic acid metabolic pathway and lysine biosynthesis metabolic pathway.关键词
伊犁马/能量代谢/耐力运动Key words
Yili horses/energy metabolism/endurance exercise分类
农业科技引用本文复制引用
黄金龙,王建文,任万路,曾亚琦,欧阳文,衡士兵,王彤亮,黄云江,胡傲贇,彭宣,孟军,姚新奎..不同运动性能伊犁马80 km耐力赛能量代谢特征差异研究[J].中国畜牧兽医,2025,52(11):5040-5052,13.基金项目
国家自然科学基金青年基金项目(32202667) （32202667）
新疆维吾尔自治区重大科技专项(2022A02013-1) （2022A02013-1）
中央引导地方科技发展资金项目(ZYYD2025JD02) （ZYYD2025JD02）
新疆维吾尔自治区自然科学基金青年科学基金(2024D01B40) （2024D01B40）
新疆马繁育与运动生理重点实验室项目(XJMFY202401) （XJMFY202401）
2024年自治区级大学生创新项目(dx5cx2024207) （dx5cx2024207）
