中国畜牧兽医2025，Vol.52Issue(11)：5087-5099,13.DOI:10.16431/j.cnki.1671-7236.2025.11.007
金钗石斛多糖对竹乡鸡盲肠菌群的影响研究
Effects of Dendrobium nobile Polysaccharide on Cecal Microbiot of Zhuxiang Chickens
摘要
Abstract
[Objective]This study aimed to investigate the effects of Dendrobium nobile polysaccharide on the cecal microbiota of Zhuxiang chickens,providing a theoretical basis for analyzing the mechanism of the polysaccharide-gut microbiota-host health interaction.[Method]Sixty 6-month-old Zhuxiang chickens were randomly divided into control(CK)and polysaccharide(DNP)groups,with 5 replicates per group(6 chickens each).Chickens in CK group were fed basal diet,while chickens in DNP group were fed a diet supplemented with 800 mg/kg Dendrobium nobile polysaccharides.After 60 days of feeding,one chicken per replicate had been selected,and their cecal contents were collected for 16S rRNA high-throughput sequencing.Alpha diversity,principal coordinate analysis(PCoA),taxonomic composition,differential species,and functional prediction analysis were performed.[Result]There was no significant difference of Alpha diversity index between DNP and CK groups(P＞0.05).PERMANOVA analysis results indicated that compared with control group,Dendrobium nobile polysaccharides treatment had a significant effect on the formation of the intestinal microbiota in Zhuxiang chickens(P＜0.05).In the gut microbiota of Zhuxiang chickens,there were six dominant phyla at the phylum level,such as Bacteroidota and Firmicutes,and 23 dominant genera at the genus level,such as Bacteroides and Rikenellaceae RC9_gut_group.Compared with control group,Dendrobium nobile polysaccharide treatment significantly increased the abundance of beneficial bacteria such as Christensenellaceae_R-7_group and UCG-005 in cecal microorganisms of Zhuxiang chickens(P＜0.05),and significantly decreased the abundance of potentially harmful bacteria such as Enterococcus and Campylobacter(P＜0.05).KEGG pathway annotation results showed that the cecal microbiota of Zhuxiang chickens were mainly enriched in metabolic pathways.[Conclusion]Dendrobium nobile polysaccharides could regulate the cecal microbiota structure of Zhuxiang chickens.By increasing the abundance of beneficial bacteria such as norank_o_Clostridia_vadinBB60_group and reducing the abundance of harmful bacteria such as Enterococcus,it could optimize the intestinal microecological balance and promote the healthy growth of Zhuxiang chickens.关键词
竹乡鸡/金钗石斛多糖/16S rRNA/盲肠菌群Key words
Zhuxiang chickens/Dendrobium nobile polysaccharide/16S rRNA/cecal microbiota分类
畜牧业引用本文复制引用
谭启松,李辉,龙霞,杨金林,冯港,周北大..金钗石斛多糖对竹乡鸡盲肠菌群的影响研究[J].中国畜牧兽医,2025,52(11):5087-5099,13.基金项目
贵州省科技厅农业领域重点项目"贵州地方鸡种质资源挖掘与新品种(配套系)选育"(黔科合支撑[2022]重点034) （配套系）
贵州省农业农村厅"贵州生态肉鸡优势特色产业集群项目"(黔财农[2024]80号) （黔财农[2024]80号）
