中国烟草科学2025，Vol.46Issue(5)：1-9,9.DOI:10.13496/j.issn.1007-5119.2025.05.001
抗青枯病雪茄烟ZX145的SNP指纹图谱构建
SNP Fingerprint Construction and Bacterial Wilt Resistance Identification of Cigar Tobacco ZX145
摘要
Abstract
To identify high-quality cigar tobacco germplasm resistant to bacterial wilt(Ralstonia solanacearum)and provide molecular markers for subsequent breeding,this study utilized GBS(genotyping-by-sequencing)resequencing data from 19 tobacco germplasm resources,including the cigar tobacco line ZX145.The number of identified ZX145-specific SNP loci was 221.The specific SNP loci were screened for the development of KASP(kompetitive allele-specific PCR)molecular markers.Validation using 71 tobacco germplasm resources of diverse types yielded 28 KASP marker pairs capable of distinguishing ZX145 from other germplasm.A specific SNP fingerprint for ZX145 was constructed using 24 loci selected to cover all chromosomes.Field evaluations for bacterial wilt resistance,alongside comprehensive assessments of agronomic and yield traits,were conducted in disease nurseries of Fujian,Guizhou,and Anhui Province.The results showed that ZX145 exhibited moderately resistance-resistance in the disease nurseries,which was superior to the conventional control varieties,and its output value performed better.Consequently,ZX145 represents an elite resistant germplasm resource for breeding bacterial wilt-resistant cigar tobacco cultivars.The developed KASP markers provide robust molecular tools to support its utilization in breeding programs.关键词
青枯病/雪茄烟/指纹图谱/抗病性Key words
bacterial wilt/cigar tobacco/fingerprint map/disease resistance分类
农业科学引用本文复制引用
李光灿,耿锐梅,修启贵,宋志美,胡海洲,杨爱国,张玉芹,曹长代,张兴伟,肖志亮,杨晴,周东新,章慧芬,周永..抗青枯病雪茄烟ZX145的SNP指纹图谱构建[J].中国烟草科学,2025,46(5):1-9,9.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC01) （ASTIP-TRIC01）
中国烟草总公司重大专项项目[110202201008(JY-08)] （JY-08）
中国烟草总公司山东省公司科技重点项目(KN304-202303) （KN304-202303）
中国烟草总公司福建省公司科技计划项目(2024350000240021) （2024350000240021）
