中国烟草科学2025，Vol.46Issue(5)：10-19,10.DOI:10.13496/j.issn.1007-5119.2025.05.002
晾晒烟腺毛分泌物抗病毒活性成分鉴定与评价
Identification and Evaluation of Antiviral Components in Glandular Trichome Secretions of Sun/Air-cured Tobacco
摘要
Abstract
In order to elucidate the control effect of glandular trichome secretions from sun/air-cured tobacco on plant viral diseases and to identify superior active components,this study assessed the anti-TMV activity of ethanol extracts from glandular trichomes of 28 sun/air-cured tobacco germplasm resources using a sap rubbing-inoculation method.In addition,the composition and content of diterpenoids and sucrose esters(SEs)in the glandular trichome secretions were determined by ultra-high performance liquid chromatography(UPLC)and gas chromatography-mass spectrometry(GC-MS).Correlation,partial least squares,and clustering analyses were used to investigate the relationship between the content of glandular trichome secretions and the anti-TMV activity.The results showed that:(1)The ethanolic extracts of glandular trichomes from 19 germplasm samples exhibited antiviral activity against TMV,with Q16(Meitan sun-cured tobacco)showing the best virucidal effect,inhibiting necrotic spots by 68.87%;(2)There was a significant or extremely significant negative correlation between cis-abienol and α-cembratriene-diol,β-cembratriene-diol,SEI,and SEII.Conversely,cis-abienol showed a significant or extremely significant positive correlation with SEIII,SEIV,and SEV.More importantly,cis-abienol,SEIII,and SEIV exhibited a highly significant positive correlation with the inhibition rate of necrotic spots.(3)Cis-abienol,SEIII,and SEIV were identified as the major active components against TMV in the glandular trichome secretions of sun/air-cured tobacco.Additionally,the validation experiments showed that cis-abienol(200 μg/mL)and SEs(300 μg/mL)exhibited the most antiviral activities,with necrosis inhibition rate of 44.32%and 53.19%respectively.These rates were significantly higer than those achieved by a plant-derived pesticide(0.5%amino-oligosaccharides).The research results provide a material foundation and technical support for the efficient utilization and the development of plant-derived antiviral active components.关键词
晾晒烟/腺毛分泌物/TMV/活性成分Key words
sun/air-cured tobacco/glandular trichome secretions/TMV/active components分类
农业科学引用本文复制引用
谭娅婷,刘艳华,亓超凡,杜咏梅,张洪博,杨颖,韩晓,付秋娟,欧阳一鸣,刘克强..晾晒烟腺毛分泌物抗病毒活性成分鉴定与评价[J].中国烟草科学,2025,46(5):10-19,10.基金项目
黑龙江烟草工业有限责任公司项目(202400000034004) （202400000034004）
中国农业科学院科技创新工程项目(ASTIP-TRIC05) （ASTIP-TRIC05）
