中国烟草科学2025，Vol.46Issue(5)：20-27,8.DOI:10.13496/j.issn.1007-5119.2025.05.003
日灼对烟草叶片叶绿素荧光诱导动力学特征的影响
Effects of Sunburn on Chlorophyll Fluorescence Induction Kinetics Characteristics in Tobacco Leaves
摘要
Abstract
In order to explore the effects of sunburn on photosynthetic structure and function of tobacco leaves,and analyze its occurrence and response mechanisms,this study used flue-cured tobacco Cuibi No.1 as the experimental material.Sunburn was simulated by sudden strong light after shading and water spraying,mimicking abrupt sunny weather following prolonged overcast and rainy conditions.Rapid chlorophyll fluorescence induction kinetics analysis technology was used to explore the change of chlorophyll fluorescence characteristics in tobacco leaves during the important period from the occurrence of sunburn to short-term recovery(T0,normal growth;T1,high humidity and few light;T2,sudden strong light;T3,12 h after sunburn;T4,24 h after sunburn).The results showed that sunburn caused significant deformation of the OJIP curve during the T1-T4 period,with the curve tending to linearity by T4,accompanied by a notable decrease in the peak of fluorescence intensity and obvious variations in the O-P phase segments.During T2 period,Fm and QYmax decreased significantly,while Fo,VJ and Mo increased significantly.The increase of light energy utilization followed the order:DIo/RC>ABS/RC>TRo/RC>ETo/RC.Additionally,ΦP,ΦE and ψo decreased significantly,whereas ψD increased significantly.Ac increased significantly,with the increase in Dd exceeding that of Tr.The environmental stress sensitivity parameter PIABS was significantly reduced by 70.40%.During the T3-T4 period,the related fluorescence parameters showed a trend of recovery or mitigation toward normal growth,but PIABS continued to decrease.This suggests that sunburn has caused serious irreversible damage to the photosynthetic structure of tobacco leaves.In the short-term recovery response process,chlorophyll fluorescence parameters showed obvious environmental adaptability changes:by reversibly inactivating reaction center,the absorption and quantum transfer of light energy are reduced to avoid the accumulation of excess excitation energy.Concurrently,the distribution ratio of light energy utilization was adjusted,and the heat dissipation was increased on the basis of reducing light energy capture,thereby enhancing resistance to sunburn damage.关键词
烟草/日灼/叶绿素荧光参数/光合能力Key words
tobacco/sunburn/chlorophyll fluorescence parameters/photosynthetic capacity分类
农业科学引用本文复制引用
袁斌,王雪仁,陈承亮,陈涛,吴建华,林智慧,宋文静..日灼对烟草叶片叶绿素荧光诱导动力学特征的影响[J].中国烟草科学,2025,46(5):20-27,8.基金项目
中国烟草总公司福建省公司科技项目(2024350000240017) （2024350000240017）
