中国烟草科学2025，Vol.46Issue(5)：53-60,8.DOI:10.13496/j.issn.1007-5119.2025.05.007
云南植烟土壤青枯菌定量检测及病害风险分析
Quantitative Detection and Risk Analysis of Ralstonia solanacearum Species Complex in Tobacco-planting Soils from Yunnan Province
摘要
Abstract
The purposes of this research were to investigate the relationship between the pre-planting soil abundance of Ralstonia solanacearum species complex(RSSC)and the occurrence of tobacco bacterial wilt,as well as to analyze the distribution patterns of RSSC in the soils of tobacco planting areas from Yunnan Province.We used a previously established qPCR detection system to quantitatively detect RSSC in the soil before transplanting flue-cured tobacco and examined the incidence of tobacco bacterial wilt during the rapid growth stage to the maturity stage of tobacco.The results showed that:(1)The population threshold of the pathogens causing tobacco bacterial wilt was 103 to 104 genomes per gram of soil.(2)The population of RSSC showed a falling trend with soil depth,mainly concentrated in the soil layer within 40 cm.(3)Analysis of 716 soil samples revealed that samples with RSSC contents in the range of 104 to 105 genomes per gram of soil accounted for the most significant proportion(37.43%).Among these,Baoshan,Kunming,and Lincang had relatively high proportions(58.33%,51.43%,and 45.25%,respectively).Five cities,including Dali,Zhaotong,Wenshan,Chuxiong,and Yuxi,had the highest proportion of samples with less than 103 genomes per gram,which were 62.50%,61.54%,56.04%,48.48%,and 41.67%respectively.(4)The abundance of RSSC in soil samples from gramineous crops in spring rotation were significantly lower than those from continuously cropped fields.Compared with winter fallow,the contents of RSSC in soils planted with crops from the families of Solanaceae,Polygonaceae,and Cruciferaceae were higher.Conversely,the contents of RSSC in soils cultivated with crops from the families of Leguminosae and Asteraceae were lower,and only former plants significantly reduced the RSSC in soils.According to the incidence threshold of 103～104 RSSC genomes/g in the soil,the risk of bacterial wilt in Kunming,Lincang,Pu'er,Qujing,Honghe,and Baoshan of Yunnan Province is relatively high,and attention should be paid to prevention and control.关键词
烟草青枯病/qPCR/土壤检测/发病阈值/过程控制内参Key words
tobacco bacterial wilt/qPCR/soil detection/disease risk threshold/internal sample process controls分类
植物保护学引用本文复制引用
李善魁,夏振远,晋艳,姜文君,张力群,卢灿华,陈伟,马俊红,王继明,胡加云,户艳霞,李江舟,盖晓彤,姜宁..云南植烟土壤青枯菌定量检测及病害风险分析[J].中国烟草科学,2025,46(5):53-60,8.基金项目
中国烟草总公司云南省公司科技计划重点项目(2024530000241008、2023530000241014) （2024530000241008、2023530000241014）
云南省基础研究计划项目面上项目(202301AT070508、YNDG202302YY01) （202301AT070508、YNDG202302YY01）
国家自然科学基金地区基金(32260702、32272611) （32260702、32272611）
