中国烟草科学2025，Vol.46Issue(5)：77-86,10.DOI:10.13496/j.issn.1007-5119.2025.05.010
高产α-淀粉酶基因工程菌A8-1的发酵优化及应用
Fermentation Optimization and Application of High-Yield α-amylase Genetically Engineered Bacterium Strain A8-1
摘要
Abstract
In order to improve the α-amylase production activity of genetically engineered strain BAX-5/PT-17 SP002amy(A8-1),improve its industrial applicability and application effect in tobacco processing,a series of parameters in the fermentation process were optimized through single factor test and response surface experiments,and the scaled-up process was validated in a fermenter.Meanwhile,changes in chemical composition and sensory quality of tobacco with α-amylase treatment were analyzed.After optimization,the optimal fermentation medium and conditions for strain fermentation were determined as:corn meal 65 g/L,soybean cake meal 70 g/L,NaCl 10 g/L,CaCl2 3 g/L,fermentation time 52 h,temperature 40℃,pH 6.0,inoculation volume 3%.The optimized α-amylase activity reached 1880.1 U/mL.Compared with 30.6 U/mL before optimization,the enzyme activity increased by 61.4-fold changes,and the enzyme activity could achieve 2276.2 U/mL in 5 L fermenter.After 7 U/g α-amylase treatment,the starch content of tobacco decreased by 19.9%,the total sugar and reducing sugar content increased by 8.09%and 7.81%,respectively.The aroma precursor substances and other indicators were positively improved in varying degrees.The treated tobacco exhibited enhanced aroma richness,smoother and milder smoke,with reduced irritancy and off-notes.The optimized fermentation process significantly improved the enzyme yield of genetically engineered strain A8-1,demonstrating strong industrial application potential.Its implementation in cigarette production effectively modifies chemical composition and elevates smoke quality.关键词
α-淀粉酶/发酵/烟草/应用Key words
α-amylase/fermentation/tobacco/application分类
轻工业引用本文复制引用
张泽锟,段晓姣,叶长文,安璐,吴爽爽,李力群,席红如,郝捷,牛晨启..高产α-淀粉酶基因工程菌A8-1的发酵优化及应用[J].中国烟草科学,2025,46(5):77-86,10.基金项目
中国烟草总公司重点研发项目(110202102019) （110202102019）
