中国烟草科学2025，Vol.46Issue(5)：87-98,12.DOI:10.13496/j.issn.1007-5119.2025.05.011
烟草内生贝莱斯芽孢杆菌的筛选鉴定及在烟叶发酵中的应用
Screening and Identification of Endophytic Bacillus Velezensis in Tobacco and Its Application in Tobacco Leaf Fermentation
摘要
Abstract
In order to clarify the biochemical functions of tobacco endophytic bacteria and explore their application in tobacco leaf fermentation,a strain NS42 with high-efficiency in degrading proteins,cellulose,starch,lignin,and pectin was isolated from fresh tobacco plants.Based on morphological characteristics and phylogenetic tree analysis,NS42 was preliminarily identified as Bacillus velezensis.Following fermentation with NS42,the chemical composition of tobacco leaves was modulated,leading to an enhancement in the aroma quality of flue-cured tobacco leaves.Notably,both the aroma intensity and sweetness of flue-cured tobacco leaves increased,while irritation and offensive odors were diminished,resulting in a more pleasant aftertaste.The reducing sugar content increased by 18.51%.The contents of starch,cellulose,lignin,protein,and pectin in tobacco leaves decreased by 15.61%,9.67%,8.49%,12.70,and 16.47%,respectively.The content of aroma compounds including 5-methylfurfural,β-dihydrodamascenone,megastigmatrienone,farnesylacetone,β-ionone,solanone,phenylethyl alcohol,benzaldehyde,and neophytadiene were significantly elevated.The whole genome of NS42 comprises 6885670540 base pairs encoding 3919 genes,among which 7 genes were related to protein degradation,6 to cellulose degradation,6 to starch degradation,9 to pectin degradation,and 4 to lignin degradation.This strain can utilize various polysaccharides and proteins as carbon and nitrogen sources.In this study,an endophytic Bacillus strain with robust degrading enzyme activity was isolated and characterized,demonstrating a significant impact on the chemical aroma components and sensory quality of tobacco leaves,thereby showcasing substantial potential for enhancing tobacco leaf quality.关键词
烟草内生菌/烟叶发酵/香味成分/烟叶提质Key words
tobacco endophytes/tobacco aging/aroma component/quality improvement of tobacco leaves分类
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王祯,刘晨,张建栋,宋凯,魏进彬,杨红静,臧志鹏,高玉珍,毛多斌..烟草内生贝莱斯芽孢杆菌的筛选鉴定及在烟叶发酵中的应用[J].中国烟草科学,2025,46(5):87-98,12.基金项目
中国烟草总公司科技重点研发项目(110202202006) （110202202006）
中国烟草实业发展中心科技项目(ZYSYQ-2022-4) （ZYSYQ-2022-4）
甘肃省重点研发计划(22YF11GA292) （22YF11GA292）
