中国药房2025，Vol.36Issue(21)：2638-2644,7.DOI:10.6039/j.issn.1001-0408.2025.21.04
基于AIM2焦亡途径探讨化瘀解毒方减轻慢性肾脏病炎性损伤的作用机制
Mechanism of Huayu jiedu formula in alleviating inflammatory injury in chronic kidney disease based on AIM2 pyroptosis pathway
摘要
Abstract
OBJECTIVE To explore the mechanism of Huayu jiedu formula in regulating inflammatory injury in chronic kidney disease(CKD).METHODS Fifty male SD rats were randomly divided into a sham surgery group(10 rats)and a modeling group(40 rats).The CKD model was replicated in the modeling group by unilateral ureteral obstruction surgery.After successful modeling,the rats were randomly divided into model group,esaxerenone group(positive control),and TCM low-and high-dose groups,with 10 rats in each group.The Esaxerenone group was given 1 mg/kg of esaxerenone,while the TCM low-and high-dose groups were given 13.7 and 27.4 g/kg of Huayu jiedu formula respectively,the sham surgery group and model group were given an equal volume of physiological saline,all groups were intervened continuously for 14 days.Hematoxylin eosin and Masson staining were used to observe the pathological changes in rat kidney tissue.Conventional biochemical methods were used to detect serum urea(SUr),serum creatinine(SCr),malondialdehyde(MDA),and superoxide dismutase(SOD)levels;enzyme-linked immunosorbent assay was used to detect the levels of serum interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α);immunohistochemistry and Western blot were used to detect the protein expression of peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α),mitochondrial transcription factor A(TFAM),absent in melanoma 2(AIM2),caspase-1,gasdermin D(GSDMD),IL-1β and IL-18 in renal tissue;real-time fluorescence quantitative PCR was used to detect the mRNA expression of AIM2.RESULTS Compared with the sham surgery group,the renal tissue of the model group showed pathological changes such as glomerular deformation and destruction,severe tubular dilation,and increased deposition of blue fibrin;the levels of SUr,SCr,MDA,IL-1β,IL-6,TNF-α,the protein expression of AIM2,GSDMD,caspase-1,IL-1β,IL-18,and the mRNA expression of AIM2 were significantly increased or up-regulated(P＜0.01);the levels of SOD,the protein expression of PGC-1α,TFAM were significantly reduced or down-regulated(P＜0.01).Compared with the model group,all treatment groups showed improvement in the above symptoms and most indicators in rats.CONCLUSIONS Huayu jiedu formula may improve renal function,alleviate renal inflammatory damage and pyroptosis,and exert renal protective effects by regulating the AIM2 pyroptosis pathway.关键词
慢性肾脏病/化瘀解毒方/黑色素瘤缺乏因子2/焦亡途径Key words
chronic kidney disease/Huayu jiedu formula/absent in melanoma 2/pyroptosis pathway分类
医药卫生引用本文复制引用
薛瑾环,武子雯,杨帆,楼芸芸,丁英钧,肖郁朋,刘籼慧,梁文杰..基于AIM2焦亡途径探讨化瘀解毒方减轻慢性肾脏病炎性损伤的作用机制[J].中国药房,2025,36(21):2638-2644,7.基金项目
河北省自然科学基金项目(No.H2022423356) （No.H2022423356）
国家自然科学基金项目(No.82305121) （No.82305121）
