中国中西医结合儿科学2025，Vol.17Issue(5)：402-409,后插1,9.DOI:10.20274/j.cnki.issn.1674-3865.2025.05.008
五虎汤对呼吸道合胞病毒诱导的外泌体引起哮喘小鼠气道炎症的干预作用研究
Investigating the therapeutic role of Wuhu decoction for airway inflammation in mice with asthma caused by RSV-induced exosomes
摘要
Abstract
Objective To investigate the effects of Wuhu decoction on airway inflammation-related indicators in asthmatic mice induced by respiratory syncytial virus(RSV)-primed bone marrow mesenchymal stem cell-derived exosomes(BMSCs-Exo-RSV).Methods The asthma mouse model was established by intranasal administration of BMSCs-Exo-RSV.Sixty SPF-grade male C57 mice were randomly divided into six groups(n=10 per group):control group,BMSCs-Exo-RSV model group,ribavirin intervention group,and low-,medium-,and high-dose Wuhu decoction intervention groups.Interventions were performed and recorded once daily for two weeks.After the experiment,the behaviors of the mice in each group were compared.Pulmonary function testing using the DSI Buxco PFT system was conducted to measure residual volume/total lung capacity ratio(RV/TLC)and airflow limitation of the pulmonary function of mice;histopathological changes of lung tissues were compared via Masson and HE staining;ELISA quantification of IFN-γ,IL-12,IL-4,and IL-6 levels in bronchoalveolar lavage fluid(BALF)was performed;qPCR was used to determine microRNA-144(miRNA-144)expression.Results Compared with the control group,BMSCs-Exo-RSV model mice exhibited clinical symptoms(tachypnea,nasal scratching,sneezing,weight loss),elevated RV/TLC(P<0.01)that indicated airflow limitation,aggravated inflammatory injury of lung tissues by pathological examination,decreased IFN-γ and IL-12(P<0.01)and increased IL-4 and IL-6(P<0.01)in BALF that further suggested formation of airway inflammation,and upregulated miRNA-144(P<0.01).Compared with model group,Wuhu decoction(all doses)and ribavirin groups showed improvement in clinical symptoms of tachypnea,nasal scratching,sneezing and weight loss,airflow limitation was reduced(P<0.01),pulmonary inflammation was improved,IFN-γ and IL-12(P<0.01)increased while IL-4 and IL-6 decreased(P<0.01)in BALF,and the expression of miRNA-144 decreased(P<0.01).Conclusion Wuhu decoction effectively modulates immune balance and alleviates airway inflammation in BMSCs-Exo-RSV-induced asthmatic mice,which may be related to its inhibition of miRNA-144 upregulation.关键词
哮喘/气道炎症/五虎汤/外泌体/骨髓间充质干细胞/miRNA/小鼠Key words
Asthma/Airway inflammation/Wuhu decoction/Exosomes/Bone marrow mesenchymal stem cells(BMSCs)/miRNA/Mice分类
医药卫生引用本文复制引用
周智,王孟清,罗银河,姚冰,刘靖雷,王国红,李雪怡,马啸..五虎汤对呼吸道合胞病毒诱导的外泌体引起哮喘小鼠气道炎症的干预作用研究[J].中国中西医结合儿科学,2025,17(5):402-409,后插1,9.基金项目
国家自然科学基金项目(82174437) （82174437）
全国名老中医药专家传承工作室(国中医药人教函[2022]75号) National Natural Science Foundation of China(No.82174437) （国中医药人教函[2022]75号）
