中国医药科学2025，Vol.15Issue(19)：24-27,50,5.DOI:10.20116/j.issn2095-0616.2025.19.05
烟酰胺磷酸核糖转移酶抑制剂FK866通过抑制炎症反应减轻LPS诱导的新生小鼠急性肺损伤
Nicotinamide phosphoribosyltransferase inhibitor FK866 mitigates LPS-induced acute lung injury in neonatal mice by suppressing inflammatory response
摘要
Abstract
Objective This study investigates whether the nicotinamide phosphoribosyltransferase(NAMPT)inhibitor FK866 can mitigate systemic inflammatory responses and lung injury using a lipopolysaccharide(LPS)-induced acute lung injury(ALI model in neonatal mice.Methods Neonatal C57BL/6J male mice were randomly divided into four groups using a random number table method:control group,FK866 group,LPS group,and FK866+LPS group.The control group received intraperitoneal injections of equivalent saline on days 5-7.The FK866 and FK866+LPS groups were pretreated with 10 mg/(kg·d)FK866 via intraperitoneal injection for two consecutive days starting on day 5.The LPS and FK866+LPS groups received 10 mg/kg LPS intraperitoneally on day 7 to induce systemic inflammation and ALI.All neonatal mice were sacrificed on day 8 for collection of lung tissues and serum.Results Hematoxylin-eosin(HE)staining revealed significantly higher lung injury scores in the LPS group compared to the control group(P<0.05).The FK866+LPS group showed significantly lower lung injury scores than the LPS group(P<0.05).Serum levels of inflammatory factors including tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,and IL-6 were significantly elevated in the LPS group compared to controls(P<0.05),while these inflammatory markers were significantly reduced in the FK866+LPS group compared to the LPS group(P<0.05).Conclusion FK866 alleviates systemic inflammation and inflammatory-associated ALI in a neonatal mouse model of sepsis-related ALI,suggesting that NAMPT inhibitors may play a protective role in mitigating neonatal acute lung injury.关键词
烟酰胺磷酸核糖转移酶/全身炎症反应综合征/急性肺损伤/炎症因子Key words
Nicotinamide phosphoribosyltransferase/Systemic inflammatory response syndrome/Acute lung injury/Inflammatory factor分类
药学引用本文复制引用
Abelita,PSPM Ximenes,雷晓鹏,毕广良..烟酰胺磷酸核糖转移酶抑制剂FK866通过抑制炎症反应减轻LPS诱导的新生小鼠急性肺损伤[J].中国医药科学,2025,15(19):24-27,50,5.基金项目
广东省自然科学基金(2022A1515012021). （2022A1515012021）
