中国医药科学2025，Vol.15Issue(19)：71-75,5.DOI:10.20116/j.issn2095-0616.2025.19.15
顶空-气相色谱-离子迁移谱结合电子眼鉴别细辛及其形似伪品徐长卿
Identification of Asari Radix et Rhizoma and its morphologically similar counterfeit Cynanchi Paniculati Radix et Rhizoma by HS-GC-IMS combined with an E-eye
摘要
Abstract
Objective To resolve the confusion arising from the morphological similarity between Asari Radix et Rhizoma(ARR)and Cynanchi Paniculati Radix et Rhizoma(CPRR),we developed a dual-modal identification system integrating headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS)and electronic eye(E-eye)technologies.By analyzing differences in odor and color characteristics,accurate identification was achieved,thereby ensuring medication safety.Methods HS-GC-IMS and E-eye were integrated with principal component analysis(PCA),discriminant function analysis(DFA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)to characterize volatile components and color profiles.Results A total of 143 components were identified by HS-GC-IMS.ARR contained 41 characteristic components(terpenes,esters,and pyrazines),while CPRR contained 22 characteristic components(sulfur-containing compounds,aldehydes,and heterocyclic compounds).Thirty-six differential markers with variable importance in projection(VIP)>1 were screened out by OPLS-DA.The E-eye analysis revealed that ARR was predominantly composed of yellow and brown tones,accompanied by small amounts of orange and light greenish-yellow,while CPRR primarily featured yellow and brown as base colors,supplemented with pale olive tones,greenish-yellow,and yellowish-pink.The study showed that ARR exhibited higher brightness with prominent yellow-blue tonal characteristics,whereas CPRR demonstrated significant red-green tonal attributes.The PCA-DFA model successfully achieved a 100%identification accuracy rate in distinguishing between these two herbal medicines.Conclusion This study defined discriminative indicators in volatile compounds and color features between ARR and CPRR using HS-GC-IMS and E-eye,providing a novel technical strategy for precise authentication of morphologically similar herbs.关键词
细辛/徐长卿/顶空-气相色谱-离子迁移谱/电子眼/挥发性成分Key words
Asari Radix et Rhizoma/Cynanchi Paniculati Radix et Rhizoma/HS-GC-IMS/E-eye/Volatile organic compounds分类
医药卫生引用本文复制引用
张芯茹,陈月华,刘芳彤,张慧,康廷国,宋慧鹏..顶空-气相色谱-离子迁移谱结合电子眼鉴别细辛及其形似伪品徐长卿[J].中国医药科学,2025,15(19):71-75,5.基金项目
辽宁省科技计划联合计划项目(2024-MSLH-297) （2024-MSLH-297）
辽宁省沈阳市科技人才专项项目(U35拔尖青年项目)(RC230846) （U35拔尖青年项目）
辽宁省教育厅高校基本科研项目储备项目(2024-JYTCB-080). （2024-JYTCB-080）
