中国真菌学杂志2025，Vol.20Issue(5)：449-455,7.DOI:10.3969/j.issn.1673-3827.2025.05.001
小鼠血浆和组织中两性霉素B的液相色谱-串联质谱法浓度测定
Determination the concentration of amphotericin B in mouse plasma and tissues by liquid chromatography-mass spectrometry
摘要
Abstract
Objective To establish a liquid chromatography-mass spectrometry(LC-MS/MS)method for the deter-mination of the concentration of the antifungal drug amphotericin B(AmB)in mouse plasma and tissues.Methods Using liver as representative tissue sample,the protein precipitation method was used for sample pretreatment of mouse plasma and liver.Chromatographic separation was performed using a shim-pack GIST-HP C18 AQ(2.10 mm×50.00 mm,3.00 μm)chromatographic column.Gradient elution was performed using water containing 2.00 mmol/L ammonium acetate and 0.10%formic acid as mobile phase A,and acetonitrile containing 0.10%formic acid as mobile phase B(sample vol-ume was 5.00 μL,mobile phase flow rate was 0.45 mL/min,column temperature was 40℃).The electrospray ion source was set up detection conducted in positive ion mode and multiple reaction monitoring mode.The quantitative ion pairs of AmB and the internal standard natamycin were m/z 924.50→906.50 and m/z 666.50→503.40,respectively.Re-sults The response of this LC-MS/MS method to interfering components was less than 10.00%of the response at the lower limit of quantification of the analyte,demonstrating good specificity.The lower limit of quantification for AmB and the internal standard detection in both plasma and tissue was 0.1 μg/mL,with good linearity in the range of 0.10 μg/mL-100.00 μg/mL,accuracies(recoveries)of intra-day and inter-day were all more than 95.00%,and the relative standard deviations(RSD)of precision,matrix effect,and dilution effect were all less than 10.00%.AmB could be stored stably under avoid light and low-temperature conditions.Conclusions The LC-MS/MS method for determining AmB concentra-tion in mouse plasma and tissues was successfully established,and all investigation results meet the methodological re-quirements.关键词
两性霉素B/小鼠/药物浓度/液相色谱-串联质谱Key words
amphotericin B/mice/drug concentration/LC-MS/MS引用本文复制引用
蔡刘阳,何海洋,张莉,廖万清,薛潇春,潘炜华..小鼠血浆和组织中两性霉素B的液相色谱-串联质谱法浓度测定[J].中国真菌学杂志,2025,20(5):449-455,7.基金项目
国家自然科学基金面上项目(82472304) （82472304）
国家自然科学基金青年项目(82304037) （82304037）
上海市长宁区卫健委课题(20234Y039) （20234Y039）
