摘要
Abstract
Objective:To investigate the effects of germacrone(GM)regulation of the cyclic GMP-AMP synthase(cGAS)-stimulator of interferon gene(STING)signaling pathway on the proliferation,migration,invasion and apoptosis of prostate cancer(PCa)cells.Methods:PCa PC3 cells were randomly separated into the control group(normal culture),the L-GM,the M-GM,the H-GM groups(each treated with 120,240,480 μmol/L GM respectively),and the GM+RU.521 group(treated with 480 μmol/L GM+1 µmol/L cGAS inhibitor RU.521).EdU staining,CCK-8 assay,scratch assay,Transwell assay,and flow cytometry were applied to detect the effects of GM on the proliferation,migration,invasion,and apoptosis of PC3 cells,respectively.WB assay was used to detect the effects of GM on the expressions of cGAS and STING proteins in PC3 cells.Results:Compared with those in the control group,the rate of EdU-positive cells,cell proliferation activity,scratch healing rate,and the number of invasive cells in the L-GM,M-GM,and H-GM groups were significantly reduced(all P<0.05);the apoptosis rate increased(P<0.05);the expressions of cGAS and STING proteins were significantly upregulated,and were concentration-dependent(all P<0.05).Compared with those in the H-GM group,the rate of EdU positive cells,cell proliferation activity,scratch healing rate and the number of invasive cells in the GM+RU.521 group were significantly elevated(all P<0.05);the cell apoptosis rate decreased(P<0.05);and the expressions of cGAS and STING proteins were significantly downregulated(all P<0.05).Conclusion:GM inhibits the proliferation,migration,invasion and promotes the apoptosis of PCa cells by activating the cGAS-STING signaling pathway.关键词
吉马酮/环鸟苷酸-腺苷酸合成酶/干扰素基因刺激因子/前列腺癌/PC3细胞/增殖/迁移/侵袭/凋亡Key words
germacrone(GM)/cyclic GMP-AMP synthase(cGAS)/stimulator of interferon gene(STING)/prostate cancer(PCa)/PC3 cell/proliferation/migration/invasion/apoptosis分类
医药卫生
