中国中医急症2025，Vol.34Issue(10)：1571-1576,6.DOI:10.3969/j.issn.1004-745X.2025.10.002
锦红汤治疗脓毒症急性肺损伤作用机制的网络药理学和分子对接研究
Network Pharmacology and Molecular Docking Study on the Mechanism of Jinhong Decoction in Treating Acute Lung Injury Induced by Sepsis
摘要
Abstract
Objective:To explore the mechanism of Jinhong Decoction in treating acute lung injury(ALI)in-duced by sepsis using network pharmacology and molecular docking techniques.Methods:Active compounds and targets of Jinhong Decoction were identified through databases including TCMSP,SwissADME,UniProt,GeneCards,OMIM,as well as literature mining.An"herb-compound-target"network was constructed using Cytoscape 3.9.1.Venn diagrams were used to identify key overlapping targets between Jinhong Decoction and sepsis-induced ALI.Protein-protein interaction(PPI)networks were analyzed via the STRING 11.5 database.Gene Ontology(GO)en-richment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses were performed using the DA-VID database.Molecular docking between active components of Jinhong Decoction and core targets was validated and visualized using the TCMSPAS system and Pymol software.Results:A total of 144 potential targets of Jin-hong Decoction and 1 416 disease-related targets for sepsis-induced ALI were identified.Twenty-three common targets were obtained through intersection analysis,with core targets including TNF,IL-1β,TP53,CXCL8,MAPK3,and Alb.Enrichment analysis revealed 364 GO terms and 106 KEGG pathways.Key signaling pathways included the MAPK,Toll-like receptor,IL-17,and TNF signaling pathways.Molecular docking results indicated strong binding affinities between the main target TNF and active compounds such as chrysin,luteolin,and querce-tin.Conclusion:Jinhong Decoction exerts therapeutic effects on sepsis-induced ALI through multiple compo-nents,targets,and pathways,providing a theoretical basis and reference for clinical prevention and treatment.关键词
脓毒症急性肺损伤/网络药理学/分子对接/锦红汤Key words
Sepsis-induced acute lung injury/Network pharmacology/Molecular docking/Jinhong Decoction分类
医药卫生引用本文复制引用
陈采玉,屠亦文,张文,彭伟,张春蕾,王蓓,方邦江..锦红汤治疗脓毒症急性肺损伤作用机制的网络药理学和分子对接研究[J].中国中医急症,2025,34(10):1571-1576,6.基金项目
国家自然科学基金项目(82374350) （82374350）
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023067) （zyyzdxk-2023067）
国家重点研发计划中医药现代化项目(2024YFC3505700) （2024YFC3505700）
中国中医优势专科建设项目(2024YSZKZZYX006) （2024YSZKZZYX006）
上海中医药大学第三轮"学术荣誉体系"讲席教授(中医人字[2024]38号) （中医人字[2024]38号）
