中国中医急症2025，Vol.34Issue(10)：1603-1607,5.DOI:10.3969/j.issn.1004-745X.2025.10.008
参附注射液联合标准操作程序对脓毒症休克大鼠心肌抑制作用的研究
Study on the Myocardial Inhibitory Effects of Shenfu Injection Combined with Standard Operating Procedure in Rats with Septic Shock
摘要
Abstract
Objective:To investigate the mechanism of Shenfu injection combined with standard operating proce-dure(SOP)in alleviating myocardial inhibition in rats with septic shock.Methods:60 Sprague-Dawley(SD)rats were randomly divided into five groups,12 in each group.A myocardial inhibition model was established by intra-peritoneal injection of lipopolysaccharide(LPS)at a dose of 10 mg/kg.Model success was confirmed by measuring serum levels of cardiac troponin l(cTnl)and N-terminal pro-B-type natriuretic peptide(NT-proBNP),as well as by echocardiographic assessment.The successfully modeled rats were then randomly assigned to the sham group,model group,Shenfu group,SOP group,or Shenfu+SOP group,12 in each group.Hematoxylin-eosin(HE)stain-ing was used to evaluate myocardial pathological changes.Enzyme-linked immunosorbent assay(ELISA)was per-formed to measure serum levels of NT-proBNP,interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).West-ern blotting was used to detect protein expression of phosphorylated p38 mitogen-activated protein kinase(p-p38MAPK)and total p38MAPK.Results:After 3 and 6 h of treatment,the heart rate(HR),respiratory rate(R),and mean arterial pressure(MAP)in the Shenfu+SOP group were significantly improved compared with the mod-el group and the Shenfu group(P<0.05).After 6 h of treatment,the IL-6 levels in the Shenfu+SOP group were significantly lower than those in the SOP group(P<0.05).Severe inflammatory reactions,including myocardial de-generation and necrosis,were observed in all septic shock groups.Electron microscopy revealed nuclear pyknosis,reduced mitochondria,and interstitial fibrosis in cardiomyocytes.The expression levels of p-p38MAPK and p38MAPK in the Shenfu+SOP group showed statistically significant differences compared with the SOP group at the same time points(P<0.05).Conclusion:Shenfu injection combined with SOP can effectively alleviate myocar-dial inhibition in rats with septic shock.关键词
脓毒症/脓毒症休克/参附注射液/心肌抑制/标准操作程序/大鼠Key words
Sepsis/Septic shock/Shenfu injection/Myocardial inhibition/Standard operating procedure/Rats分类
医药卫生引用本文复制引用
李青霞,杨妍,郭树明,倪震博,田苗..参附注射液联合标准操作程序对脓毒症休克大鼠心肌抑制作用的研究[J].中国中医急症,2025,34(10):1603-1607,5.基金项目
甘肃省卫生健康行业科研计划项目(GSWSKY2023-50) （GSWSKY2023-50）
