中国畜禽种业2025，Vol.21Issue(10)：27-31,5.
运动对绵羊腹腔镜活体采卵-体外胚胎生产效率的影响
Effect of Exercise on the Efficiency of Laparoscopic Live Egg Harvesting-in vitro Embryo Production in Sheep
摘要
Abstract
In this experiment,55 Blackhead Suffolk adult ewes in good condition were randomly divided into a forced exercise group and a control group,with 29 ewes in the exercise group and 26 ewes in the non-exercise group.In the compulsory exercise group,sheep were exercised for 1 hour per day over a distance of 3.5 km for 21 days per month for 3 months,while in the control group,sheep were only allowed to move freely in the pen every day.Eggs were harvested in vivo by laparoscopy and then matured in vitro,fertilized in vitro and cultured in embryo culture.The average total number of eggs recovered from oocytes,the average number of available eggs,the average rate of available eggs,the 24-h cleavage rate,the total cleavage rate,the rate of D6 blastocysts,as well as the rate of embryo development after fertilization of the oocytes(number of D6 blastocysts/number of fertilized eggs)were analyzed.The results showed that:exercise did not affect the average total number of eggs recovered,the average number of available eggs and the rate of available eggs(P＞0.05);the cleavage rate of embryos in the exercise group was greater than that in the non-exercise group,but the difference was not significant(P＞0.05);and the rate of embryo development after fertilization of oocytes in the exercise group was significantly greater than that in the control group(P＜0.01).It can be seen that exercise can improve the developmental ability of oocytes and increase the efficiency of in vitro embryo production.关键词
运动/绵羊/卵母细胞/体外成熟/体外胚胎生产Key words
Exercise/Sheep/Oocyte/In vitro maturation/In vitro embryo production分类
农业科技引用本文复制引用
李蓉,陈大勇,程磊,席广银,李明扬,曹苗,郭倚天,范利宏,包梅荣,仇春娟,龚璐,乌日嘎..运动对绵羊腹腔镜活体采卵-体外胚胎生产效率的影响[J].中国畜禽种业,2025,21(10):27-31,5.基金项目
科技创新2030"农业生物育种"重大项目(2022ZD04013、2023ZD04075) （2022ZD04013、2023ZD04075）
内蒙古自治区中央引导地方科技发展资金(2024ZY0160) （2024ZY0160）
内蒙古自治区科技突围重大项目(2024). （2024）
