中国猪业2025，Vol.20Issue(5)：14-23,10.DOI:10.16174/j.issn.1673-4645.2025.05.006
藏猪精液品质特性及精液冷冻程序优化探索
Exploration of the Quality Characteristics of Tibetan Pig Semen and Optimization of Frozen Semen Cryopreservation Procedures
摘要
Abstract
The study focused on the quality of Tibetan pig semen and the optimization of the frozen semen process,aimed to improve the conservation efficiency and development value of Tibetan pig resources.Using Tibetan pig semen as the research object,the effects of seasonal factors on semen quality were systematically analyzed,and freezing and thawing procedures were optimized.The results showed that semen quality in spring and autumn was significantly better than in summer and winter.For the thawing procedure,the high-temperature rapid thawing method at 47 ℃ for 15 seconds was the most effective,with post-thaw sperm viability reaching 68.03％and motility 54.94％,and viability remained above 30％after storage at 17 ℃ for 24 hours.Further research found that during freezing,using a fine tube distance of 4 cm from the liquid nitrogen surface significantly improved post-thaw sperm viability and motility com-pared to 5 cm.Based on the characteristics of Tibetan pig semen,it was recommended to use a freezing procedure with a fine tube distance of 4 cm from the liquid nitrogen surface,combined with a high-temperature rapid thawing method at 47 ℃ for 15 seconds,to achieve better post-thaw sperm quality.关键词
藏猪/精液品质/冷冻精液/解冻程序/保种/精子活力/开发利用Key words
Tibetan pig/semen quality/frozen semen/thawing procedure/breed conservation/sperm motility/exploitation and utilization分类
畜牧业引用本文复制引用
邹宇,朱砺,甘麦邻,曹宇豪,廖荣杰,胡世杰,周立新,薛佳,吴启杭,邹莹洁,沈林園..藏猪精液品质特性及精液冷冻程序优化探索[J].中国猪业,2025,20(5):14-23,10.基金项目
国家生猪产业技术体系项目(CARS-35) （CARS-35）
国家现代农业产业技术体系四川生猪创新团队项目(SCCXTD-2025-8) （SCCXTD-2025-8）
四川省科技计划项目(2021YFYZ0007) （2021YFYZ0007）
