高脂饲料诱导的肥胖猪模型代谢性炎症与系统性免疫稳态失衡研究OA

To explore the association between metabolic inflammation and systemic immune homeostasis imbalance in obese pig models induced by a high-fat feed,this study selected 24 healthy 40 day old white pigs and randomly divided them into two groups,the control group(fed a basal feed)and the high-fat group(fed a high-fat feed),with 12 pigs in each group.The experiment lasted for 12 weeks.Western blot was used to detect the expression levels of inflammation-related proteins(NF-κB p65,IκBα)in adipose tissue.Quantita-tive real-time polymerase chain reaction(qRT-PCR)was employed to determine the relative mRNA expression levels of pro-inflamma-tory factors(TNF-α,IL-6,IL-1 β)and anti-inflammatory factors(IL-10,TGF-β)in peripheral blood mononuclear cells,liver,and adi-pose tissue.The results showed that compared with the control group,the final body weight,Lee's index,and fat coefficient of pigs in the high-fat group were significantly increased(P＜0.05);the phosphorylation level of NF-κB p65 in adipose tissue was significantly in-creased(P＜0.05),while the protein expression level of IκBα was significantly decreased(P＜0.05);the mRNA expression levels of TNF-α,IL-6,and IL-1β in peripheral blood mononuclear cells,liver and adipose tissue were significantly up-regulated(P＜0.05),whereas the mRNA expression levels of IL-10 and TGF-β were significantly down-regulated(P＜0.05).In conclusion,pigs with obesity induced by a high-fat feed exhibit obvious metabolic inflammatory responses,and the mechanism may be related to the activation of the NF-κB signaling pathway.Meanwhile,they were accompanied by systemic immune homeostasis imbalance,which was manifested as an imbalanced state of high expression of pro-inflammatory factors and low expression of anti-inflammatory factors.This study pro-vided an ideal large animal model basis for the research on the pathogenesis and intervention of obesity-related metabolic diseases.